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Rapid multiplex PCR assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary samples: A 30-minute assay
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-11-12 , DOI: 10.1016/j.mimet.2020.106103 Takeshi Inoue 1 , Yoshitomo Chihara 1 , Keisuke Kiba 1 , Shuya Hirao 1 , Masahiro Tanaka 1 , Tatsuo Yoneda 1 , Toshihisa Saka 1 , Ken-Ichi Beppu 2 , Keiichi Furubayashi 3 , Mizuki Takashima 4 , Hidenori Nagai 4 , Hiroshi Kobayashi 5 , Kiyohide Fujimoto 1
中文翻译：
用于同时检测泌尿生殖系统样本中淋病奈瑟菌和沙眼衣原体的快速多重 PCR 检测：30 分钟检测
更新日期：2020-12-08
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2020-11-12 , DOI: 10.1016/j.mimet.2020.106103 Takeshi Inoue 1 , Yoshitomo Chihara 1 , Keisuke Kiba 1 , Shuya Hirao 1 , Masahiro Tanaka 1 , Tatsuo Yoneda 1 , Toshihisa Saka 1 , Ken-Ichi Beppu 2 , Keiichi Furubayashi 3 , Mizuki Takashima 4 , Hidenori Nagai 4 , Hiroshi Kobayashi 5 , Kiyohide Fujimoto 1
Affiliation
We developed a rapid multiplex PCR assay available in bedside for the simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis, which enables diagnosis in less than 30 minutes. In this study, we validated the clinical utility of this assay including its sensitivity and specificity for NG and CT detection.
中文翻译：
用于同时检测泌尿生殖系统样本中淋病奈瑟菌和沙眼衣原体的快速多重 PCR 检测：30 分钟检测
我们开发了一种可在床边同时检测淋病奈瑟菌和沙眼衣原体的快速多重 PCR 检测方法，可在30 分钟内完成诊断。在这项研究中，我们验证了该测定的临床实用性，包括其对 NG 和 CT 检测的敏感性和特异性。
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