Recombinant hybrid antibodies are commonly used in antigen-targeting assays to reduce the immunogenic potential associated with using classic mouse antibodies in other species. The DEC205 receptor has become an attractive target due to its effectiveness in activating the immune response and is considered a promising vaccination target. The aim of this study was to produce a fully chimeric mouse x pig anti-porcine DEC205 recombinant antibody (rAb). Based on a mouse anti-porcine DEC205 monoclonal antibody (mAb), we designed and expressed a chimeric mouse x pig rAb using the Expi293f system. The resulting rAb maintained the recognition capacity of the native mouse mAb toward the porcine DEC205 receptor, as evidenced by western blot analysis. By using flow cytometry, we evaluated the ability of the rAb to recognize DEC205⁺ dendritic cells. In conclusion, the chimeric mouse x pig anti-DEC205 rAb can be used in antigen-targeting assays as a vaccination strategy in pigs.

重组杂交抗体通常用于抗原靶向测定，以降低与在其他物种中使用经典小鼠抗体相关的免疫原性潜力。DEC205 受体因其在激活免疫反应方面的有效性而成为一个有吸引力的目标，并被认为是一个有希望的疫苗接种目标。本研究的目的是生产完全嵌合的小鼠 x 猪抗猪 DEC205 重组抗体 (rAb)。基于小鼠抗猪 DEC205 单克隆抗体 (mAb)，我们使用 Expi293f 系统设计并表达了嵌合小鼠 x 猪 rAb。蛋白质印迹分析证明，所得的 rAb 保持了天然小鼠 mAb 对猪 DEC205 受体的识别能力。通过使用流式细胞术，我们评估了 rAb 识别 DEC205 ⁺树突状细胞。总之，嵌合小鼠 x 猪抗 DEC205 rAb 可用于抗原靶向测定，作为猪的疫苗接种策略。