Four nematophagous fungi (NF) were identified by traditional and molecular techniques, and their in vitro predatory activity (PA) against Haemonchus contortus (Hc), and Panagrellus redivivus (Pr) was assessed. Additionally, the nematocidal activity (NA) of fungal organic extracts (OEs) obtained from a methanol/dichloromethane system was assessed. Fungi were grown in PDB medium under shaking (SchC) and static conditions (StatC). The PA of Arthrobotrys conoides (Ac), A. oligospora (Ao), A. arthrobotryoides (Aa) and Purpureocillium lilacinum (Pl) against Hc were 75, 71, 66 and 52%, respectively. Meanwhile, the PA against Pr for same fungal genera/species were 99.8, 99.9, 99.4 and 96.3%, respectively. The highest NA of the OEs were 89.5, 81.6 and 72.1% for Aa, Ac and Ao, respectively, under StatC. Moreover, Pl cultured under SchC caused 78% Hc larval mortality. The four assessed NF and their OEs are promising candidates as potential biocide agents against parasitic nematodes.

通过传统和分子技术鉴定了四种线虫真菌（NF），并评估了它们对弯曲变形杆菌（Hc）和重生帕纳格雷（Panagrellus redivivus）（Pr）的体外捕食活性（PA）。此外，评估了从甲醇/二氯甲烷系统获得的真菌有机提取物（OEs）的杀线虫活性（NA）。真菌在摇动（SchC）和静态条件（StatC）下在PDB培养基中生长。的PA节丛conoides（AC），A. oligospora（奥），A. arthrobotryoides（了Aa）和Purpureocillium lilacinum（PL）对Hc分别为75％，71％，66％和52％。同时，相同真菌属/种对Pr的PA分别为99.8％，99.9、99.4和96.3％。在StatC下，Aa，Ac和Ao的OE的最高NA分别为89.5％，81.6和72.1％。而且，在SchC下培养的P1引起78％的Hc幼虫死亡率。经过评估的四个NF及其OEs有望成为潜在的杀灭寄生线虫的杀生物剂。