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Perfluorooctanoic acid-induced cellular and subcellular alterations in fish hepatocytes
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-11-12 , DOI: 10.1016/j.etap.2020.103548
Maurizio Manera , Giuseppe Castaldelli , Elisa A. Fano , Luisa Giari

Liver perfluorooctanoic acid (PFOA) pathophysiology and related morphofunction disturbances were studied in common carp at the cellular and subcellular level and with box-counting fractal analysis of ultrathin sections to assess the effect of PFOA exposure on hepatocyte structure complexity and heterogeneity. Three experimental groups were investigated: unexposed; low exposure (200 ng L−1 PFOA); high exposure (2 mg L−1 PFOA). PFOA-exposed cells showed differences from controls at both tested concentrations, manifested mainly as cloudy swelling and reversible vacuolar degeneration. Subcellular modifications primarily involved mitochondria and secondarily endoplasmic reticulum, with evidence of increased subcellular turnover. The alterations were consistent with oxidative stress related pathophysiology. Fractal analysis discriminated exposed from unexposed fish and low from high PFOA exposure based on lacunarity and fractal dimension, respectively. The absence of irreversible organelle alterations and apoptosis/necrosis, along with the increase of cellular complexity, led to the conclusion that the patterns observed represented an adaptive recovery response.



中文翻译:

全氟辛酸诱导鱼肝细胞的细胞和亚细胞改变

在细胞和亚细胞水平研究鲤鱼全氟辛酸(PFOA)的病理生理学和相关的形态功能障碍,并对超薄切片进行盒计数分形分析,以评估PFOA暴露对肝细胞结构复杂性和异质性的影响。研究了三个实验组:未暴露;未暴露;未暴露。低暴露(200 ng L -1 PFOA);高暴露(2 mg L -1PFOA)。在两种测试浓度下,暴露于PFOA的细胞均与对照细胞存在差异,主要表现为浑浊肿胀和可逆液泡变性。亚细胞修饰主要涉及线粒体,其次是内质网,具有亚细胞更新增加的证据。改变与氧化应激相关的病理生理学一致。分形分析分别根据盲点和分形维数来区分未暴露鱼的暴露和低PFOA的暴露。缺乏不可逆的细胞器改变和凋亡/坏死,以及细胞复杂性的增加,得出结论,观察到的模式代表了适应性恢复反应。

更新日期:2020-11-22
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