Several studies have used zebrafish to investigate the effects of environmental enrichment on behavior and physiology. However, to date there are no studies evaluating the behavioral responses, such as habituation and exploration, of enriched-housed zebrafish when they are submitted to novelty paradigms. The present work was, therefore, designed to evaluate the habituation and exploratory responses of zebrafish exposed to enriched- (EE) and non-enriched (NE) environments when they face novelty. Adult wild-type zebrafish were used. Three different enriched contexts were designed. In Context 1, zebrafish was exposed to enrichment during 7 days, which reduced their total distance traveled in novel tank and social preference tests in comparison to the non-enriched animals. In Context 2, animals were exposed to same enrichment during 14 days. EE exposure did not alter the behavioral responses of zebrafish compared to NE. In Context 3, fish were exposed to enrichment during 14 days, with changing the enriching elements at day 8. Similarly to Context 1, total distance traveled was reduced by EE exposure when compared to NE. Our results suggest a modulatory effect of EE on adult zebrafish locomotion that may be dependent on the time of exposure and on the physical structure of the enriched environment.

中文翻译：

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在新型水族箱范式中，较短和较长时间的环境富集对成年斑马鱼探索活动（Danio rerio）的不同影响

几项研究使用斑马鱼来研究环境富集对行为和生理的影响。然而，迄今为止，还没有研究评估富集斑马鱼在接受新范式时的行为反应，例如习惯和探索。因此，目前的工作旨在评估斑马鱼在面对新鲜事物时暴露于富集 (EE) 和非富集 (NE) 环境中的习惯化和探索性反应。使用成年野生型斑马鱼。设计了三种不同的丰富上下文。在上下文 1 中，斑马鱼在 7 天内暴露于富集，与未富集的动物相比，这减少了它们在新型坦克和社会偏好测试中的总距离。在上下文 2 中，动物在 14 天内暴露于相同的富集。与 NE 相比，EE 暴露并没有改变斑马鱼的行为反应。在情景 3 中，鱼在 14 天期间暴露于富集，在第 8 天改变富集元素。与情景 1 类似，与 NE 相比，EE 暴露减少了总行驶距离。我们的结果表明 EE 对成年斑马鱼运动的调节作用可能取决于暴露时间和丰富环境的物理结构。