Significance: High temporal stability, wavelength independency, and scalable field of view (FOV) are the primary requirements of a quantitative phase microscopy (QPM) system. The high temporal stability of the system provides accurate measurement of minute membrane fluctuations of the biological cells that can be an indicator of disease diagnosis. Aim: The main aim of this work is to develop a high temporal stable technique that can accurately quantify the cell’s dynamics such as membrane fluctuations of human erythrocytes. Further, the technique should be capable of acquiring scalable FOV and resolution at multiple wavelengths to make it viable for various biological applications. Approach: We developed a single-element nearly common path, wavelength-independent, and scalable resolution/FOV QPM system to obtain temporally stable holograms/interferograms of the biological specimens. Results: With the proposed system, the temporal stability is obtained ∼15 mrad without using any vibration isolation table. The capability of the proposed system is first demonstrated on USAF resolution chart and polystyrene spheres (4.5-μm diameter). Further, the system is implemented for single shot, wavelength-independent quantitative phase imaging of human red blood cells (RBCs) with scalable resolution using color CCD camera. The membrane fluctuation of healthy human RBCs is also measured and was found to be around 47 nm. Conclusions: Contrary to its optical counterparts, the present system offers an energy efficient, cost effective, and simple way of generating object and reference beam for the development of common-path QPM. The present system provides the flexibility to the user to acquire multi-wavelength quantitative phase images at scalable FOV and resolution.

中文翻译：

---

高度时间稳定、波长独立、可扩展的视场共径定量相位显微镜

意义：高时间稳定性、波长独立性和可扩展视场 (FOV) 是定量相位显微镜 (QPM) 系统的主要要求。该系统的高时间稳定性可准确测量生物细胞的微小膜波动，这可以作为疾病诊断的指标。目的：这项工作的主要目的是开发一种高时间稳定的技术，可以准确量化细胞的动态，如人类红细胞的膜波动。此外，该技术应该能够在多个波长下获得可扩展的 FOV 和分辨率，使其适用于各种生物应用。方法：我们开发了一种单元素几乎通用的路径，与波长无关，和可扩展的分辨率/FOV QPM 系统，以获得生物标本的时间稳定全息图/干涉图。结果：使用所提出的系统，在不使用任何隔振表的情况下获得了约 15 mrad 的时间稳定性。拟议系统的能力首先在美国空军分辨率图和聚苯乙烯球体（4.5 微米直径）上得到证明。此外，该系统使用彩色 CCD 相机实现了具有可扩展分辨率的人类红细胞 (RBC) 的单次、波长独立的定量相位成像。还测量了健康人红细胞的膜波动，发现大约为 47 nm。结论：与其光学对应物相反，本系统提供了一种节能、经济、以及生成目标和参考光束的简单方法，用于开发共路径 QPM。本系统为用户提供了以可缩放的 FOV 和分辨率获取多波长定量相位图像的灵活性。