The evolution of single-stranded DNA (ssDNA) assembly on octadecylamine (ODA) modified highly oriented pyrolytic graphite (HOPG) surface by heating and ultrasonic treatment has been studied for the first time. We have observed that DNA on the ODA coated HOPG surface underwent dramatic morphological changes as a function of heating and ultrasonic treatment. Ordered DNA firstly changed to random aggregates by heating and then changed to three-dimensional (3D) networks by ultrasonic treatment. This finding points to previously unknown factors that impact graphite-DNA interaction and opens new opportunities to control the deposition of DNA onto graphitic substrates. In this way, we built a cost-effective method to produce large-scale 3D ssDNA networks. All of these studies pave the way to understand the properties of DNA-solid interface, design novel nanomaterials, and improve the sensitivity of DNA biosensors.

中文翻译：

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加热超声处理十八胺改性石墨表面DNA重排

首次研究了通过加热和超声处理在十八胺（ODA）改性的高取向热解石墨（HOPG）表面上单链DNA（ssDNA）组装的演变。我们观察到 ODA 涂层 HOPG 表面上的 DNA 在加热和超声处理的作用下经历了显着的形态变化。有序DNA首先通过加热变为随机聚集体，然后通过超声处理变为三维（3D）网络。这一发现指出了以前未知的影响石墨-DNA 相互作用的因素，并为控制 DNA 在石墨基底上的沉积开辟了新的机会。通过这种方式，我们构建了一种经济高效的方法来生成大规模 3D ssDNA 网络。所有这些研究都为理解 DNA-固体界面的特性铺平了道路，