Purpose: To investigate the comparative effectiveness of certain biological injectable stimulants for the healing of skin wounds and criteria for its assessment.
Materials and Methods: A comparative study of the effectiveness of mesenchymal stem cells (SC group), collagen (Collagen group), and deproteinized calf blood hemoderivative (DCBH group) was carried out using an acute wound model. Control wounds were injected with isotonic sodium chloride solution (Control group). A total of four groups (28 wounds per group) were included in the study. Aged male Wistar rats were used as experimental animals. A dynamic assessment of the wound areas and edges, microvasculature assessment via laser Doppler flowmetry, histological and morphometric analyses to determine the quantitative and qualitative fibroblasts composition, as well as the degree of newly synthesized collagen maturity, was conducted on days 0, 3, 7, and 14.
Results: The administration of SCs provided a rapid but short-lasting effect, whereas the administration of collagen resulted in a delayed but long-lasting wound-healing effect. DCBH resulted in little to no effect. An increase in the perfusion volume of the wound edges accelerated the regeneration process, while the level of microcirculation did not affect the number and activity of fibroblasts. The wound healing acceleration, as well as the new collagen and stratified epithelium formation and maturation, was associated with the presence of a sufficient pool of mature and active fibroblasts in the wound, and not with the number of fibroblasts.
Conclusion: The present results clarify the action mechanisms of the studied drugs. In addition, the application purposes and different effects of each drug on the different wound healing phases were demonstrated. An assumption on the multi-component treatment advisability under the wound condition objective assessment possibility was made. Findings from this study may assist clinicians in making an informed transition to personalized wound management and achieve better clinical outcomes.

Keywords: collagen, deproteinized calf blood hemoderivative, mesenchymal stem cell, fibroblast, microhemocirculation, wound


中文翻译：

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几种生物注射型伤口愈合刺激剂的效果对比分析及评价标准


目的：研究某些生物注射兴奋剂对皮肤伤口愈合的比较效果及其评估标准。

材料和方法：使用急性伤口模型对间充质干细胞（SC组）、胶原蛋白（Collagen组）和去蛋白小牛血血液衍生物（DCBH组）的有效性进行比较研究。对照伤口注射等渗氯化钠溶液（对照组）。该研究总共包括四组（每组 28 个伤口）。使用老年雄性Wistar大鼠作为实验动物。在第 0、3、7 天对伤口区域和边缘进行动态评估，通过激光多普勒血流计进行微脉管系统评估，组织学和形态测量分析以确定定量和定性成纤维细胞组成，以及新合成的胶原蛋白成熟度。和 14。

结果： SCs 的施用提供了快速但持续时间短的效果，而胶原蛋白的施用则导致了延迟但持久的伤口愈合效果。 DCBH 几乎没有效果。伤口边缘灌注量的增加加速了再生过程，而微循环水平并不影响成纤维细胞的数量和活性。伤口愈合加速以及新胶原和复层上皮的形成和成熟与伤口中存在足够的成熟且活跃的成纤维细胞有关，而不是与成纤维细胞的数量有关。

结论：目前的结果阐明了所研究药物的作用机制。此外，还论证了每种药物在不同伤口愈合阶段的应用目的和不同作用。根据伤口状况客观评估的可能性，对多成分治疗的合理性做出了假设。这项研究的结果可以帮助临床医生明智地过渡到个性化伤口管理并取得更好的临床结果。


Keywords:胶原蛋白, 小牛血去蛋白血液衍生物, 间充质干细胞, 成纤维细胞, 微血液循环, 伤口