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Production of isobutyric acid from methanol by Clostridium luticellarii
Green Chemistry ( IF 9.3 ) Pub Date : 2020-10-29 , DOI: 10.1039/d0gc02700f Camille Petrognani 1, 2, 3, 4, 5 , Nico Boon 1, 2, 3, 4, 5 , Ramon Ganigué 1, 2, 3, 4, 5
Green Chemistry ( IF 9.3 ) Pub Date : 2020-10-29 , DOI: 10.1039/d0gc02700f Camille Petrognani 1, 2, 3, 4, 5 , Nico Boon 1, 2, 3, 4, 5 , Ramon Ganigué 1, 2, 3, 4, 5
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Renewable methanol can be used as a feedstock to generate value-added multicarbon components through fermentation technologies. Recently, researchers reported the production of isobutyric acid using methanol as an electron donor with open culture systems dominated by Eubacterium sp. and Clostridium sp. Here we report the ability of Clostridium luticellarii wild-type strain to produce isobutyric acid from methanol and CO2 & H2. When growing on methanol, the supplementation of acetic and butyric acid enhanced isobutyric acid final concentration, selectivity and production rate. A maximum of 5.04 ± 0.08 g L−1 isobutyric acid was produced at a rate of 0.420 ± 0.012 g L−1 d−1and selectivity of 0.70 electron per electron of total products in batch with acetic and butyric acid as electron acceptors. The pH was also found to be a major factor influencing isobutyric acid formation with maximal production at pH 6.50. Finally, in addition to its ability to produce isomers, C. luticellarii was able to perform C2-unit elongation from methanol. Overall, this study positions C. luticellarii as a promising platform for the production of isocarboxylic acids.
中文翻译：
产黄梭菌由甲醇生产异丁酸
可再生甲醇可用作原料，通过发酵技术产生增值的多碳组分。最近，研究人员报道了使用以甲醇作为电子供体的异丁酸生产，该开放供体的培养系统以真杆菌属为主。和梭菌属。在这里，我们报告了梭状芽孢杆菌野生型菌株从甲醇，CO 2和H 2产生异丁酸的能力。在甲醇上生长时，补充乙酸和丁酸可提高异丁酸的终浓度，选择性和生产率。最多以0.420±0.012 g L -1的速率生成5.04±0.08 g L -1异丁酸d -1，以乙酸和丁酸为电子受体时，总产物的电子选择性为0.70电子。还发现pH是影响异丁酸形成的主要因素，在pH 6.50下其最大产量。最后，除了其生产异构体的能力之外，卢氏梭菌还能够从甲醇中进行C2单元延伸。总的来说，这项研究将卢氏梭状芽孢杆菌定位为生产异羧酸的有前途的平台。
更新日期：2020-11-12
中文翻译：
产黄梭菌由甲醇生产异丁酸
可再生甲醇可用作原料，通过发酵技术产生增值的多碳组分。最近，研究人员报道了使用以甲醇作为电子供体的异丁酸生产，该开放供体的培养系统以真杆菌属为主。和梭菌属。在这里，我们报告了梭状芽孢杆菌野生型菌株从甲醇，CO 2和H 2产生异丁酸的能力。在甲醇上生长时，补充乙酸和丁酸可提高异丁酸的终浓度，选择性和生产率。最多以0.420±0.012 g L -1的速率生成5.04±0.08 g L -1异丁酸d -1，以乙酸和丁酸为电子受体时，总产物的电子选择性为0.70电子。还发现pH是影响异丁酸形成的主要因素，在pH 6.50下其最大产量。最后，除了其生产异构体的能力之外，卢氏梭菌还能够从甲醇中进行C2单元延伸。总的来说，这项研究将卢氏梭状芽孢杆菌定位为生产异羧酸的有前途的平台。
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