Very Long Chain Lipids Favor the Formation of a Homogeneous Phase in Stratum Corneum Model Membranes,Langmuir

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Very Long Chain Lipids Favor the Formation of a Homogeneous Phase in Stratum Corneum Model Membranes
Langmuir ( IF 3.7 ) Pub Date : 2020-11-10 , DOI: 10.1021/acs.langmuir.0c02305
Adrian Paz Ramos ₁ , Joke A Bouwstra ₂ , Michel Lafleur ₁

Affiliation

The stratum corneum (SC), the outermost layer of mammal epidermis, acts as a barrier dictating the rate of absorption of exogenous molecules through the skin, as well as to prevent excessive water loss from the body. The SC consists of protein-rich corneocytes embedded into a complex lipid mixture. The lipid fraction is mainly constituted of an equimolar mixture of ceramides (Cer), free fatty acids (FFA), and cholesterol (Chol), forming a solid phase in the intracellular space; this lipid phase is supposed to play a fundamental role in the SC barrier function. An unusual characteristic of this biological membrane is that its lipids generally bear very long acyl chains, with the 24-carbon long ones being the most abundant. In this work, we used Raman microspectroscopy and infrared spectroscopy to study the influence of the acyl chain length on the lipid mixing properties in SC model membranes. Our results revealed that the combination of ceramides and FFA bearing a very long chain is required for the formation of homogeneous lipid mixtures, while lipids with shorter chains (16-carbon and 20-carbon atom long) lead to domains with micrometer dimensions. It is proposed that the biological machinery necessary for acyl chain elongation occurring at the mammalian skin level is required to inhibit lipid phase separation, a critical feature in the proper barrier functioning.

中文翻译：

超长链脂质有利于角质层模型膜均相的形成

角质层（SC）是哺乳动物表皮的最外层，可作为屏障，指示外源性分子通过皮肤吸收的速率，并防止人体过多失水。SC由嵌入复杂脂质混合物中的富含蛋白质的角质细胞组成。脂质部分主要由神经酰胺（Cer），游离脂肪酸（FFA）和胆固醇（Chol）的等摩尔混合物组成，在细胞内空间形成固相。该脂质相应该在SC屏障功能中起基本作用。这种生物膜的一个不寻常的特征是它的脂质通常带有很长的酰基链，而24碳长的酰基链最丰富。在这项工作中我们使用拉曼光谱和红外光谱研究了酰基链长度对SC模型膜中脂质混合特性的影响。我们的结果表明，神经酰胺和带有很长链的FFA的组合对于形成均质脂质混合物是必需的，而具有较短链（长16个碳原子和20个碳原子）的脂质会导致形成微米级的区域。提出需要在哺乳动物皮肤水平发生酰基链延长所必需的生物机制来抑制脂质相分离，脂质相分离是适当的屏障功能中的关键特征。而具有较短链（长16个碳原子和20个碳原子）的脂质会形成具有微米尺寸的区域。提出需要在哺乳动物皮肤水平发生酰基链延长所必需的生物机制来抑制脂质相分离，脂质相分离是适当的屏障功能中的关键特征。而具有较短链（长16个碳原子和20个碳原子）的脂质会形成具有微米尺寸的区域。提出需要在哺乳动物皮肤水平发生酰基链延长所必需的生物机制来抑制脂质相分离，脂质相分离是适当的屏障功能中的关键特征。

更新日期：2020-11-25

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