Identification and Characterization of a Peptide from the Stony Coral Heliofungia actiniformis,Journal of Natural Products

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Identification and Characterization of a Peptide from the Stony Coral Heliofungia actiniformis
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-11-09 , DOI: 10.1021/acs.jnatprod.0c00981
Casey A Schmidt ₁ , David T Wilson ₁ , Ira Cooke _{2,

3} , Jeremy Potriquet _{1,

4} , Katie Tungatt _{1,

3} , Visai Muruganandah ₁ , Chloë Boote _{2,

3,

5} , Felicity Kuek _{2,

3,

5} , John J Miles _{1,

3} , Andreas Kupz ₁ , Stephanie Ryan ₁ , Alex Loukas ₁ , Paramjit S Bansal ₁ , Rozita Takjoo ₁ , David J Miller _{2,

3,

5} , Steve Peigneur ₆ , Jan Tytgat ₆ , Norelle L Daly ₁

Affiliation

Marine organisms produce a diverse range of toxins and bioactive peptides to support predation, competition, and defense. The peptide repertoires of stony corals (order Scleractinia) remain relatively understudied despite the presence of tentacles used for predation and defense that are likely to contain a range of bioactive compounds. Here, we show that a tentacle extract from the mushroom coral, Heliofungia actiniformis, contains numerous peptides with a range of molecular weights analogous to venom profiles from species such as cone snails. Using NMR spectroscopy and mass spectrometry we characterized a 12-residue peptide (Hact-1) with a new sequence (GCHYTPFGLICF) and well-defined β-hairpin structure stabilized by a single disulfide bond. The sequence is encoded within the genome of the coral and expressed in the polyp body tissue. The structure present is common among toxins and venom peptides, but Hact-1 does not show activity against select examples of Gram-positive and Gram-negative bacteria or a range of ion channels, common properties of such peptides. Instead, it appears to have a limited effect on human peripheral blood mononuclear cells, but the ecological function of the peptide remains unknown. The discovery of this peptide from H. actiniformis is likely to be the first of many from this and related species.

中文翻译：

来自石珊瑚 Heliofungia actiniformis 的肽的鉴定和表征

海洋生物会产生多种毒素和生物活性肽，以支持捕食、竞争和防御。尽管存在用于捕食和防御的触手可能含有一系列生物活性化合物，但石珊瑚（石珊瑚目）的肽库仍然相对不足。在这里，我们展示了来自蘑菇珊瑚Heliofungia actiniformis的触手提取物, 包含许多分子量范围类似于锥蜗牛等物种毒液分布的肽。使用 NMR 光谱和质谱，我们表征了具有新序列 (GCHYTPFGLICF) 和明确定义的 β-发夹结构的 12 残基肽 (Hact-1)，由单个二硫键稳定。该序列在珊瑚的基因组内编码并在息肉身体组织中表达。存在的结构在毒素和毒液肽中很常见，但 Hact-1 对革兰氏阳性和革兰氏阴性细菌的选择实例或一系列离子通道没有活性，这些肽的共同特性。相反，它似乎对人外周血单核细胞的影响有限，但肽的生态功能仍然未知。这种肽的发现来自H. actiniformis可能是该物种和相关物种中的第一个。

更新日期：2020-11-25

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