Biosynthesis of the Calorie-Free Sweetener Precursor ent-Kaurenoic Acid from CO2 Using Engineered Cyanobacteria,ACS Synthetic Biology

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Biosynthesis of the Calorie-Free Sweetener Precursor ent-Kaurenoic Acid from CO2 Using Engineered Cyanobacteria
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-11-11 , DOI: 10.1021/acssynbio.0c00523
Sung Cheon Ko ₁ , Han Min Woo _{1,

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Affiliation

To supply the sustainable calorie-free sweetener stevioside, synthetic photosynthetic bacteria were developed to produce ent-kaurenoic acid as a precursor of stevioside directly from CO₂. By the use of a combinatorial and modular approach for gene expression, including a cytochrome P450 and the corresponding reductase, engineered Synechoccous elongatus PCC 7942 as a model cyanobacterium enabled the biosynthesis of ent-kaurenoic acid at 2.9 ± 0.01 mg L^–1 from CO₂. We found that the order of genes for expression was critical, producing ent-kaurenoic acid by balancing gene expressions and accumulation of the toxic intermediate in a cell. The engineered bacteria allowed the complete biosynthesis of ent-kaurenoic acid, and it will be used for stevioside biosynthesis from CO₂ as a controlled fermentation.

中文翻译：

使用工程蓝藻从 CO2 生物合成无热量甜味剂前体 ent-Kaurenoic Acid

为了提供可持续的无热量甜味剂甜菊糖苷，开发了合成光合细菌以直接从 CO ₂生产对映贝壳杉烯酸作为甜菊糖苷的前体。通过使用组合和模块化方法进行基因表达，包括细胞色素 P450 和相应的还原酶，工程化的Synechoccous elongatus PCC 7942 作为模型蓝藻能够从 CO _{2 中}生物合成2.9 ± 0.01 mg L ^–1ent- kaurenoic acid . 我们发现表达基因的顺序是至关重要的，产生ent-kaurenoic acid 通过平衡基因表达和细胞中有毒中间体的积累。改造后的细菌可以完全生物合成ent- kaurenoic 酸，并将用于从 CO ₂生物合成甜菊苷作为受控发酵。

更新日期：2020-11-21

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