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Ultrasound-Assisted Interfacial Immobilization of Lipase on Hollow Mesoporous Silica Spheres in a Pickering Emulsion System: A Hyperactive and Sustainable Biocatalyst
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-11-09 , DOI: 10.1021/acssuschemeng.0c06271 Teng Sun 1 , Zhe Dong 1 , Jing Wang 1 , Feng-Hong Huang 1 , Ming-Ming Zheng 1
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-11-09 , DOI: 10.1021/acssuschemeng.0c06271 Teng Sun 1 , Zhe Dong 1 , Jing Wang 1 , Feng-Hong Huang 1 , Ming-Ming Zheng 1
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Lipase immobilization processes have the potential to drive large-scale industrial applications through cost savings. Among them, the Pickering emulsion system has recently emerged as an ecofriendly technique for enhanced enzymatic activity. In this study, a novel and facile immobilization of lipase in an ultrasound-assisted Pickering emulsion system was developed. The lipase loading amount and yield was 177.3 mg/g and 41.3%, respectively. In the enzymatic synthesis of four sterol lipoates, the immobilized lipase (UIP-HMSS@CRL) achieved the highest catalytic efficiency (CE) of 6.4 mmol/g·h among the reported methods, which was 9.1-fold higher than that of free lipase and 32.0-fold higher than that of previous methods. UIP-HMSS@CRL performed with much higher Vm and lower Km, indicating the excellent catalytic rate and stronger affinity. Moreover, UIP-HMSS@CRL displayed satisfying thermal stability and recyclability, retaining 88.6% of enzyme activity after 10 cycles. The lipase lid was opened at the oil–water interface by both ultrasound and interfacial synergistic activation effect, which provided easier access to an active site and thus achieved excellent catalytic performance. This ultrasound-assisted interface immobilization would open new opportunities for preparation of high-performance and sustainable biocatalysts.
中文翻译：
超声辅助脂肪酶在Pickering乳液系统中空介孔二氧化硅球体上的界面固定：一种高活性且可持续的生物催化剂
脂肪酶固定化工艺有潜力通过节省成本来推动大规模工业应用。其中，Pickering乳液系统最近作为提高酶活性的环保技术而出现。在这项研究中，开发了一种新型且简便的脂肪酶在超声辅助Pickering乳化体系中的固定方法。脂肪酶的负载量和产率分别为177.3mg / g和41.3％。在四种甾醇硫辛酸酯的酶促合成中，固定化脂肪酶（UIP-HMSS @ CRL）在报道的方法中达到了6.4 mmol / g·h的最高催化效率（CE），比游离脂肪酶高9.1倍。并且比以前的方法高32.0倍。UIP-HMSS @ CRL的V值更高m和更低的K m，表明优异的催化速率和更强的亲和力。此外，UIP-HMSS @ CRL表现出令人满意的热稳定性和可回收性，在10个循环后保留了88.6％的酶活性。超声和界面协同活化作用均可在油水界面处打开脂肪酶盖，从而更容易进入活性位点，从而获得出色的催化性能。这种超声辅助的界面固定将为制备高性能和可持续的生物催化剂打开新的机会。
更新日期：2020-11-09
中文翻译：
超声辅助脂肪酶在Pickering乳液系统中空介孔二氧化硅球体上的界面固定：一种高活性且可持续的生物催化剂
脂肪酶固定化工艺有潜力通过节省成本来推动大规模工业应用。其中，Pickering乳液系统最近作为提高酶活性的环保技术而出现。在这项研究中，开发了一种新型且简便的脂肪酶在超声辅助Pickering乳化体系中的固定方法。脂肪酶的负载量和产率分别为177.3mg / g和41.3％。在四种甾醇硫辛酸酯的酶促合成中，固定化脂肪酶（UIP-HMSS @ CRL）在报道的方法中达到了6.4 mmol / g·h的最高催化效率（CE），比游离脂肪酶高9.1倍。并且比以前的方法高32.0倍。UIP-HMSS @ CRL的V值更高m和更低的K m，表明优异的催化速率和更强的亲和力。此外，UIP-HMSS @ CRL表现出令人满意的热稳定性和可回收性，在10个循环后保留了88.6％的酶活性。超声和界面协同活化作用均可在油水界面处打开脂肪酶盖，从而更容易进入活性位点，从而获得出色的催化性能。这种超声辅助的界面固定将为制备高性能和可持续的生物催化剂打开新的机会。
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