In the present work, Titanium dioxide (TiO2) micro–nanostructured thin films are deposited by a cold atmospheric plasma jet on carbon fiber substrates. The surface morphology, grain size, and structure phase of TiO2 thin films are investigated by scanning electron microscopic (SEM), X-ray diffraction (XRD), and Raman spectrum. As the discharge voltage increased from 5 to 15 kV, the size of these TiO2 particles decreased from 2 to 3 μm to less than 1 μm. The XRD and Raman spectroscopic results show TiO2 on the carbon fiber surface prepared by atmospheric plasma jet is at the mixture phase of anatase and rutile. We also investigated the adhesion and proliferation assays of MC3T3-E1 preosteoblasts on the samples. The surface with smaller TiO2 particles deposited on carbon fiber is more appropriate for attachment of preosteoblasts. Furthermore, the highest proliferation of MC3T3-E1 was found on a sample with smaller TiO2 particles after incubation. Our data suggest that the increased roughness fosters cell attachment and proliferation on the surface of TiO2/carbon fibers.

在本工作中，通过冷常压等离子体喷射将二氧化钛（TiO 2）微纳米结构薄膜沉积在碳纤维基底上。通过扫描电子显微镜（SEM），X射线衍射（XRD）和拉曼光谱研究了TiO 2薄膜的表面形貌，晶粒尺寸和结构相。随着放电电压从5 kV增加到15 kV，这些TiO 2颗粒的尺寸从2到3μm减小到小于1μm。XRD和拉曼光谱结果表明TiO 2大气等离子体射流制备的碳纤维表面上的碳原子处于锐钛矿和金红石的混合相。我们还研究了MC3T3-E1前成骨细胞在样品上的粘附和增殖测定。碳纤维上沉积的TiO 2颗粒较小的表面更适合于成骨细胞的附着。此外，孵育后，在具有较小TiO 2颗粒的样品上发现了MC3T3-E1的最高增殖。我们的数据表明增加的粗糙度促进了TiO 2 /碳纤维表面上的细胞附着和增殖。