Expanding the Chemical Diversity of Genetically Encoded Libraries,ACS Combinatorial Science

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Expanding the Chemical Diversity of Genetically Encoded Libraries
ACS Combinatorial Science Pub Date : 2020-11-09 , DOI: 10.1021/acscombsci.0c00179
Sabrina E Iskandar ₁ , Victoria A Haberman ₁ , Albert A Bowers _{1,

2,

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Affiliation

The power of ribosomes has increasingly been harnessed for the synthesis and selection of molecular libraries. Technologies, such as phage display, yeast display, and mRNA display, effectively couple genotype to phenotype for the molecular evolution of high affinity epitopes for many therapeutic targets. Genetic code expansion is central to the success of these technologies, allowing researchers to surpass the intrinsic capabilities of the ribosome and access new, genetically encoded materials for these selections. Here, we review techniques for the chemical expansion of genetically encoded libraries, their abilities and limits, and opportunities for further development. Importantly, we also discuss methods and metrics used to assess the efficiency of modification and library diversity with these new techniques.

中文翻译：

扩大基因编码文库的化学多样性

核糖体的力量越来越多地被用于分子库的合成和选择。噬菌体展示、酵母展示和 mRNA 展示等技术可有效地将基因型与表型耦合，以实现许多治疗靶点的高亲和力表位的分子进化。遗传密码扩展是这些技术成功的核心，它使研究人员能够超越核糖体的内在能力，并获得新的遗传编码材料来进行这些选择。在这里，我们回顾了基因编码文库的化学扩展技术、它们的能力和局限性以及进一步开发的机会。重要的是，我们还讨论了用于评估这些新技术的修改效率和文库多样性的方法和指标。

更新日期：2020-12-14

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