Piezoelectric Heterogeneity in Collagen Type I Fibrils Quantitatively Characterized by Piezoresponse Force Microscopy,ACS Biomaterials Science & Engineering

当前位置： X-MOL 学术 › ACS Biomater. Sci. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Piezoelectric Heterogeneity in Collagen Type I Fibrils Quantitatively Characterized by Piezoresponse Force Microscopy
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-11-10 , DOI: 10.1021/acsbiomaterials.0c01314
Jinha Kwon ₁ , Hanna Cho ₁

Affiliation

Piezoelectricity of Type I collagen can provide the stress-generated potential that is considered to be one of the candidate mechanisms to explain bone’s adaptation to loading. However, it is still challenging to quantify piezoelectricity because of its heterogeneity and small magnitude. In this study, resonance-enhanced piezoresponse force microscopy (PFM) was utilized to amplify a weak piezoresponse of a single collagen fibril with a carefully calibrated cantilever. The quantitative PFM, combined with a dual-frequency resonance-tracking method, successfully identified the anisotropic and heterogenous nature of the piezoelectric properties in the collagen fibril. The profile of shear piezoelectric coefficient (d₁₅) was obtained to be periodic along the collagen fibril, with a larger value in the gap zone (0.51 pm/V) compared to the value in the overlap zone (0.29 pm/V). Interestingly, this piezoelectric profile corresponds to the periodic profile of mechanical stiffness in a mineralized collagen fibril having a higher stiffness in the gap zone. Considering that apatite crystals are nucleated at the gap zone and subsequently grown along the collagen fibril, the heterogeneous and anisotropic nature of piezoelectric properties highlights the physiological importance of the collagen piezoelectricity in bone mineralization.

中文翻译：

压电响应力显微镜定量表征I型胶原原纤维的压电异质性

I型胶原的压电性可以提供应力产生的电位，该电位被认为是解释骨骼对负荷适应的候选机制之一。然而，由于其不均一性和小幅度，量化压电性仍然具有挑战性。在这项研究中，共振增强的压电响应力显微镜（PFM）用于通过仔细校准的悬臂来放大单个胶原纤维的弱压电响应。定量PFM与双频共振跟踪方法相结合，成功地确定了胶原原纤维中压电特性的各向异性和异质性。剪切压电系数（d ₁₅）沿着胶原纤维是周期性的，与重叠区的值（0.29pm / V）相比，间隙区的值（0.51pm / V）更大。有趣的是，该压电轮廓对应于在间隙区域具有较高刚度的矿化胶原纤维的机械刚度的周期性轮廓。考虑到磷灰石晶体在间隙区成核并随后沿着胶原原纤维生长，压电特性的异质性和各向异性性质突出了胶原压电性在骨骼矿化中的生理重要性。

更新日期：2020-12-14

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11