Magnetic-Field-Assisted Cellular Osteogenic Differentiation on Magnetic Zinc Ferrite Coatings via MEK/ERK Signaling Pathways,ACS Biomaterials Science & Engineering

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Magnetic-Field-Assisted Cellular Osteogenic Differentiation on Magnetic Zinc Ferrite Coatings via MEK/ERK Signaling Pathways
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-11-09 , DOI: 10.1021/acsbiomaterials.0c01087
Bolin Tang ₁ , Xiaojun Shen ₁ , Guanchen Ye _{2,

3} , Yaru Yang ₁ , Yang Jiang ₁ , Hongqin Xia ₁ , Xiaoyi Chen _{2,

3}

Affiliation

Combining an external stimulus and stimuli-responsive biomaterials can regulate cellular behaviors. In this paper, a magneto-responsive zinc ferrite (ZnFe₂O₄) coating was designed to gain insight into the preosteoblasts behaviors and osteogenic differentiation mechanism under a static magnetic field (SMF). ZnFe₂O₄ coatings with distinct magnetization (low, medium, and high magnetizations) were prepared by being annealed at different temperatures. Cellular biology experiments indicated that all ZnFe₂O₄ coatings with the assistance of SMF could promote the early proliferation (3 days) and osteogenic differentiation of MC3T3-E1 cells. Among different ZnFe₂O₄ samples, low and medium magnetization of ZnFe₂O₄ showed a higher osteogenesis-related gene expression (Runx2, Col-I, OCN) than that of high magnetization ZnFe₂O₄ under SMF, while cellular adhesion and proliferation cultured on different ZnFe₂O₄ samples presented insignificant differences. Molecular biology tests showed that the combination of ferromagnetic ZnFe₂O₄ and SMF could significantly improve the expression level of α2β1 integrin and p-ERK. However, the addition of the inhibitor U0126 sharply reduced the expression level of p-ERK, which indicated that α2β1 integrin-mediated MEK/ERK signaling pathways play a key role in SMF-assisted cellular osteogenic differentiation over ZnFe₂O₄ coatings. This work provides an attractive strategy to enhance cellular osteogenic differentiation in a remote-control way, which exhibited enormous potential in the field of bone tissue repair and regeneration.

中文翻译：

通过 MEK/ERK 信号通路在磁性锌铁氧体涂层上的磁场辅助细胞成骨分化

结合外部刺激和刺激响应的生物材料可以调节细胞行为。在本文中，设计了一种磁响应锌铁氧体（ZnFe ₂ O ₄）涂层，以深入了解静磁场（SMF）下的前成骨细胞行为和成骨分化机制。通过在不同温度下退火制备具有不同磁化强度（低、中、高磁化强度）的ZnFe ₂ O _4涂层。细胞生物学实验表明，所有在SMF辅助下的ZnFe ₂ O ₄涂层均能促进MC3T3-E1细胞的早期增殖（3天）和成骨分化。在不同的 ZnFe ₂ O_4个样本，低磁化和中磁化ZnFe ₂ O _{4在SMF下比高磁化ZnFe}₂ O ₄的成骨相关基因表达（Runx2、Col-I、OCN）更高，而在不同ZnFe上培养的细胞粘附和增殖₂ O ₄样品呈现出微不足道的差异。分子生物学试验表明，铁磁性ZnFe ₂ O _4的结合SMF能显着提高α2β1整合素和p-ERK的表达水平。然而，抑制剂 U0126 的加入显着降低了 p-ERK 的表达水平，这表明 α2β1 整合素介导的 MEK/ERK 信号通路在 SMF 辅助的 ZnFe ₂ O ₄涂层上的细胞成骨分化中起关键作用。这项工作为远程控制增强细胞成骨分化提供了一种有吸引力的策略，在骨组织修复和再生领域表现出巨大的潜力。

更新日期：2020-12-14

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