ABSTRACT

Hepatocellular carcinoma (HCC) is one of the most aggressive malignant diseases and requires more effective prevention and treatment strategies. Mutations or overexpression of endoplasmic reticulum (ER) proteins have been frequently identified in a solid tumor, suggesting that ER proteins play an important role in tumor development. SEC61G, a component of Sec61 complex located in the membrane of the human ER, has been revealed a potential relevance in glioblastoma multiforme. Analyses from TCGA database showed that SEC61G was overexpressed in HCC. Additionally, the expression of SEC61G mRNA was associated with the survival time of HCC patients. We verified that the higher expression of SEC61G in HCC tissues than paracancerous tissues. Moreover, knockdown of SEC61G inhibited cell proliferation and induced cell apoptosis in vitro. Besides, SEC61G was required for cell migration and invasion, conferring a potential role for SEC61G in tumor transfer. Taken together, our results revealed the role of SEC61G in HCC cells. Further detailed understanding of the signaling networks underlying SEC61G involvement in HCC cells would make SEC61G as a viable therapeutic target for pharmaceutical intervention of HCC.

摘要

肝细胞癌（HCC）是最具侵袭性的恶性疾病之一，需要更有效的预防和治疗策略。在实体肿瘤中经常发现内质网 (ER) 蛋白的突变或过度表达，这表明 ER 蛋白在肿瘤发展中起重要作用。SEC61G 是位于人 ER 膜中的 Sec61 复合物的一个组成部分，已被揭示与多形性胶质母细胞瘤的潜在相关性。来自 TCGA 数据库的分析表明 SEC61G 在 HCC 中过度表达。此外，SEC61G mRNA 的表达与 HCC 患者的生存时间相关。我们证实 SEC61G 在 HCC 组织中的表达高于癌旁组织。此外，敲除 SEC61G 可抑制细胞增殖并诱导细胞凋亡体外。此外，细胞迁移和侵袭需要 SEC61G，赋予 SEC61G 在肿瘤转移中的潜在作用。总之，我们的结果揭示了 SEC61G 在 HCC 细胞中的作用。进一步详细了解 SEC61G 参与 HCC 细胞的潜在信号网络将使 SEC61G 成为 HCC 药物干预的可行治疗靶点。