ABSTRACT

Early detection and diagnosis of neurodegenerative diseases has been hampered by the lack of sensitive testing. Real-time quaking induced conversion (RT-QuIC) has been used for the early and sensitive detection of prion-induced neurologic disease, and has more recently been adapted to detect misfolded alpha-synuclein and tau as biomarkers for neurodegenerative disease. Here we use full-length recombinant tau substrates to detect tau seeding activity in Alzheimer’s disease and other human tauopathies.

中文翻译：

---

使用全长 tau 底物进行 tau 蛋白病的 RT-QuIC 检测

 抽象的

由于缺乏灵敏的检测，神经退行性疾病的早期检测和诊断受到了阻碍。实时震动诱导转换 (RT-QuIC) 已用于早期、灵敏地检测朊病毒引起的神经系统疾病，最近已用于检测错误折叠的 α-突触核蛋白和 tau 作为神经退行性疾病的生物标志物。在这里，我们使用全长重组 tau 底物来检测阿尔茨海默病和其他人类 tau 病中的 tau 播种活性。