ABSTRACT

The bacterial community structure and diversity in the rhizosphere of broomcorn millet treated with different fertilizers were analyzed. The crops were grown under one of the four fertilization treatments: M1 (compound fertilizer), M2 (farmyard manure), M3 (biological bacterial fertilizer), and M4 (no fertilizer). The soil DNA was isolated, and the 16S rRNA library was sequenced using Illumina MiSeq sequencers. The bacterial diversity and the differential bacterial genera in four groups were analyzed. Compound fertilizers had the most significant effect on plant growth, followed by farmyard manure and then biological bacterial fertilizer (p < 0.05). Actinobacteria and Pseudomonas sp. were dominant in all soil samples. The abundance of Actinobacteria was higher in M1 and M4, and the abundance of Pseudomonas sp. was higher in M3 and M4. The number of bacterial genera (208) was the highest in M1. Eight bacterial genera with low abundance were common in M1 vs. M3 and M2 vs. M3. Four bacterial genera with high richness (Phormidium sp., Cellulosimicrobium sp., Yaniella sp., and Cloacibacterium sp.) overlapped in M1 vs. M3 and in M2 vs. M3. Compound fertilizer was the most effective at encouraging broomcorn millet growth (plant height, stem diameter, panicle weight, grain weight, and yield).

摘要

分析了不同肥料处理的高粱根际细菌群落结构和多样性。作物在四种施肥处理中的一种下种植：M1（复合肥）、M2（农家肥）、M3（生物菌肥）和M4（不施肥）。分离土壤 DNA，并使用 Illumina MiSeq 测序仪对 16S rRNA 文库进行测序。分析了四组的细菌多样性和差异菌属。复合肥对植物生长的影响最大，其次是农家肥，其次是生物菌肥（p < 0.05）。放线菌和假单胞菌属。在所有土壤样品中均占优势。放线菌的丰度在 M1 和 M4 较高，而假单胞菌属 M3 和 M4 较高。细菌属的数量（208）在M1中最高。在 M1 与 M3 和 M2 与 M3 中常见 8 个低丰度细菌属。四个高度丰富的细菌属（Phormidium sp.、Cellulosimicrobium sp.、Yaniella sp.和Cloacibacterium sp.）在 M1 与 M3 和 M2 与 M3 中重叠。复合肥在促进黍子生长（株高、茎粗、穗重、粒重和产量）方面最有效。