Abstract

Follistatin (FST), a member of the transforming growth factor beta super-family regulates body growth by inhibiting the binding of myostatin (an inhibitor of growth) with its receptor in chicken. An experiment was conducted to explore ontogenic expression of the follistatin gene, determine polymorphism at the coding region of the gene and estimate its effect on growth traits in native (Aseel) and exotic broiler (PD-1) and layer (White Leghorn) chicken. The significant differences of FST gene expression were observed among the breeds revealing significantly (p < 0.05) higher expression in PD-1 line followed by White Leghorn and Aseel breeds during both embryonic and post-hatch period. The polymorphism at the functional domain of the FST gene was identified with the presence of 4 haplotypes. The follistatin haplogroups had the significant effect on body weights (p < 0.05) at 42 days of age in the White Leghorn, PD-1 and Aseel breeds (h1h1 in PD-1, h1h4 in White Leghorn and h1h2 haplogroups in Aseel breeds had the highest body weights of 770.04 ± 12.96, 246.28 ± 7.60 and 270.00 ± 10.68 g, respectively). It is concluded that the follistatin gene expressed differently during the embryonic and post-embryonic period across the breeds and the coding region of the gene was polymorphic having significant effects on growth traits in chicken.

摘要

卵泡抑素 (FST) 是转化生长因子 β 超家族的成员，它通过抑制肌肉生长抑制素（一种生长抑制剂）与其受体在鸡体内的结合来调节身体生长。进行了一项实验，以探索 follistatin 基因的个体表达，确定该基因编码区的多态性，并估计其对本地 (Aseel) 和外来肉鸡 (PD-1) 和蛋鸡 (White Leghorn) 鸡生长性状的影响。FST基因表达在品种之间存在显着差异，表明 在胚胎期和孵化后期间，PD-1 系的表达量显着高于( p < 0.05)，其次是 White Leghorn 和 Aseel 品种。FST功能域的多态性基因被鉴定为存在4个单倍型。卵泡抑素单倍群对 White Leghorn、PD-1 和Aseel品种的 42 日龄体重有显着影响（ PD-1 中的 h1h1、White Leghorn 中的 h1h4 和 Aseel 品种中的 h1h2 单倍群有最高体重分别为 770.04 ± 12.96、246.28 ± 7.60 和 270.00 ± 10.68 g）。得出的结论是，follistatin基因在胚胎期和胚胎期后不同品种的表达不同，该基因的编码区具有多态性，对鸡的生长性状有显着影响。