Gastrointestinal disease burden continues to rise in the United States and worldwide. The development of bioengineering strategies to model gut injury or disease and to re-establish functional gut tissue could expand therapeutic options and improve clinical outcomes. Current approaches leverage a rapidly evolving gut bioengineering toolkit aimed at: (i) de-novo generation of gut-like tissues at multiple scales for microtissue models or implantable grafts, and (ii) regeneration of functional gut in vivo. Although significant progress has been made in intestinal organoid cultures and engineered tissues, development of predictive in-vitro models and effective regenerative therapies remains challenging. In this review, we survey emerging bioengineering tools and recent methodological advances to identify future opportunities and challenges in gut bioengineering for disease modeling and regenerative medicine.

中文翻译：

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再生医学的肠道生物工程策略


在美国和世界范围内，胃肠道疾病负担持续上升。开发模拟肠道损伤或疾病以及重建功能性肠道组织的生物工程策略可以扩大治疗选择并改善临床结果。目前的方法利用快速发展的肠道生物工程工具包，旨在：（i）在多个尺度上从头生成肠样组织，用于微组织模型或可植入移植物，以及（ii）体内功能性肠道的再生。尽管在肠道类器官培养和工程组织方面取得了重大进展，但预测性体外模型和有效再生疗法的开发仍然具有挑战性。在这篇综述中，我们调查了新兴的生物工程工具和最新的方法学进展，以确定疾病建模和再生医学肠道生物工程的未来机遇和挑战。