Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A Genome Epidemiological Study of SARS-CoV-2 Introduction into Japan
mSphere ( IF 4.8 ) Pub Date : 2020-11-11 , DOI: 10.1128/msphere.00786-20 Tsuyoshi Sekizuka 1 , Kentaro Itokawa 1 , Masanori Hashino 1 , Tetsuro Kawano-Sugaya 1 , Rina Tanaka 1 , Koji Yatsu 1 , Asami Ohnishi 2 , Keiko Goto 3 , Hiroyuki Tsukagoshi 4 , Hayato Ehara 5 , Kenji Sadamasu 6 , Masakatsu Taira 7 , Shinichiro Shibata 8 , Ryohei Nomoto 9 , Satoshi Hiroi 10 , Miho Toho 11 , Tomoe Shimada 12 , Tamano Matsui 12 , Tomimasa Sunagawa 12 , Hajime Kamiya 12 , Yuichiro Yahata 12 , Takuya Yamagishi 12 , Motoi Suzuki 12 , Takaji Wakita 13 , Makoto Kuroda 14
mSphere ( IF 4.8 ) Pub Date : 2020-11-11 , DOI: 10.1128/msphere.00786-20 Tsuyoshi Sekizuka 1 , Kentaro Itokawa 1 , Masanori Hashino 1 , Tetsuro Kawano-Sugaya 1 , Rina Tanaka 1 , Koji Yatsu 1 , Asami Ohnishi 2 , Keiko Goto 3 , Hiroyuki Tsukagoshi 4 , Hayato Ehara 5 , Kenji Sadamasu 6 , Masakatsu Taira 7 , Shinichiro Shibata 8 , Ryohei Nomoto 9 , Satoshi Hiroi 10 , Miho Toho 11 , Tomoe Shimada 12 , Tamano Matsui 12 , Tomimasa Sunagawa 12 , Hajime Kamiya 12 , Yuichiro Yahata 12 , Takuya Yamagishi 12 , Motoi Suzuki 12 , Takaji Wakita 13 , Makoto Kuroda 14
Affiliation
After the first case of coronavirus disease 2019 (COVID-19) in Japan on 15 January 2020, multiple nationwide COVID-19 clusters were identified by the end of February. The Japanese government focused on mitigating the emerging COVID-19 clusters by conducting active nationwide epidemiological surveillance. However, an increasing number of cases continued to appear until early April 2020, many with unclear infection routes and no recent history of travel outside Japan. We aimed to evaluate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome sequences from the COVID-19 cases that appeared until early April 2020 and to characterize their genealogical networks in order to demonstrate possible routes of spread in Japan. Nasopharyngeal specimens were collected from patients, and reverse transcription-quantitative PCR tests for SARS-CoV-2 were performed. Positive RNA samples were subjected to whole-genome sequencing, and a haplotype network analysis was performed. Some of the primary clusters identified during January and February 2020 in Japan descended directly from the Wuhan-Hu-1-related isolates from China and other distinct clusters. Clusters were almost contained until mid-March; the haplotype network analysis demonstrated that the COVID-19 cases from late March through early April may have created an additional large cluster related to the outbreak in Europe, leading to additional spread within Japan. In conclusion, genome surveillance has suggested that there were at least two distinct SARS-CoV-2 introductions into Japan from China and other countries.
更新日期：2020-11-12
京公网安备 11010802027423号