Bovine milk is a complex mixture of lipids, proteins, carbohydrates, and other factors of which lipids comprise 3–5% of the total mass. Rapid analysis and characterization of the triacylglycerols (TAG) that comprise about 95% of the total lipid is daunting given the numerous TAG species. In the attached methods paper, we demonstrate an improved method for identifying and quantifying TAG species by infusion‐based “shotgun” lipidomics. Because of the broad range of TAG species in milk, a single internal standard was insufficient for the analysis and required sectioning the spectrum into three portions based upon mass range to provide accurate quantitation of TAG species. Isobaric phospholipid interferences were removed using a simple dispersive solid‐phase extraction step. Using this method, > 100 TAG species were quantitated by acyl carbon number and desaturation level in a sample of commercially purchased bovine milk.

中文翻译：

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通过输液-电喷雾质谱法对牛乳中的三酰甘油进行简单、快速的脂质组学分析

牛奶是脂质、蛋白质、碳水化合物和其他因素的复杂混合物，其中脂质占总质量的 3-5%。考虑到众多 TAG 种类，对占总脂质约 95% 的三酰甘油 (TAG) 进行快速分析和表征是一项艰巨的任务。在随附的方法论文中，我们展示了一种通过基于输注的“霰弹枪”脂质组学来识别和量化 TAG 种类的改进方法。由于牛奶中 TAG 种类的范围很广，单一的内标不足以进行分析，需要根据质量范围将光谱分成三个部分，以提供 TAG 种类的准确定量。使用简单的分散固相萃取步骤去除同量异位磷脂干扰物。使用这种方法， >