Southern corn rust (SCR), which is caused by the fungal pathogen Puccinia polysora Underw, is a prevalent foliar disease in maize. Breeding for resistant cultivars is a desirable way for the efficient control of this disease. To identify quantitative trait loci (QTL) for conferring resistance to SCR, a recombinant inbred population including 138 lines (RILs) derived from the SCR‐resistant line CML496 and susceptible line Lx9801 was evaluated for phenotypic reaction to SCR in three trials in two locations over 2 years. The population was genotyped with the maize 9.4K SNP Genotyping Array marker platform. A total of 3 QTL were mapped on chromosomes 6, 9 and 10, respectively. One major QTL on chromosome 10 (bin 10.00/10.01), RppCML496, was consistently detected across environments, which explained 43–78% of the total phenotypic variation. Using a fine mapping strategy, we delimited RppCML496 to an interval of 128 Kb. Genome mining of this region suggests two candidate genes, and a NBS‐LRR gene is the promising one for RppCML496 against SCR. The tightly linked molecular markers developed in this study can be used for molecular breeding of resistance to SCR in maize.

中文翻译：

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RppCML496的鉴定和精细定位，RppCML496是玉米中对小麦多孢菌的抗性的主要QTL

南部玉米锈病（SCR）是由真菌病原体Puccinia polysora Underw引起的，是玉米中常见的叶病。培育抗病品种是有效控制该病的理想方法。为了鉴定赋予SCR抗性的数量性状基因座（QTL），在超过两个地点的三个地点进行了三项试验，评估了包括138个SCR抗性CML496和易感性Lx9801系的重组自交群体2年。用玉米9.4K SNP基因分型阵列标记平台对种群进行基因分型。总共3个QTL分别位于6、9和10号染色体上。RppCML496是10号染色体上的一个主要QTL（bin 10.00 / 10.01）在各种环境中均能持续检测到，这解释了总表型变异的43–78％。使用精细映射策略，我们将RppCML496分隔为128 Kb的间隔。该区域的基因组挖掘表明有两个候选基因，而NBS-LRR基因是RppCML496抗SCR的有前途的基因。本研究开发的紧密连接的分子标记可用于玉米抗SCR分子育种。