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Loop-mediated isothermal amplification: Development, validation and application of simple and rapid assays for quantitative detection of species of Arcobacteraceae family- and species-specific Aliarcobacter faecis and Aliarcobacter lanthieri
Journal of Applied Microbiology ( IF 4 ) Pub Date : 2020-11-10 , DOI: 10.1111/jam.14926
I U H Khan 1 , A Becker 2 , M Cloutier 1 , M Plötz 2 , D R Lapen 1 , G Wilkes 1, 3 , E Topp 4 , A Abdulmawjood 2
Affiliation  

The family Arcobacteraceae formerly genus Arcobacter has recently been reclassified into six genera. Among nine species of the genus Aliarcobacter, Aliarcobacter faecis and Aliarcobacter lanthieri have been identified as emerging pathogens potentially cause health risks to humans and animals. This study was designed to develop/optimize, validate and apply Arcobacteraceae family- and two species-specific (A. faecis and A. lanthieri) loop-mediated isothermal amplification (LAMP) assays to rapidly detect and quantify total number of cells in various environmental niches.

中文翻译:

环介导等温扩增:开发、验证和应用简单快速的测定法,用于定量检测弧杆菌科物种和物种特异性粪杆菌和兰氏杆菌

弧菌科原属 Arcobacter最近已被重新分类为六个属。在 9 种链球菌属中,粪链球菌兰氏链球菌已被确定为新出现的病原体,可能对人类和动物造成健康风险。本研究旨在开发/优化、验证和应用 Arcobacteraceae 科和两个物种特异性(A. faecisA. lanthieri)环介导的等温扩增 (LAMP) 检测,以快速检测和量化各种环境中的细胞总数壁龛。
更新日期:2020-11-10
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