Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide‐based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid, after supplementation with the polymer FP003. A growth/death analysis revealed that 2 days of aggregate formation and 2 days of suspension in the Bingham plastic medium improved cell growth and prevented cell death. After the initial aggregation step, whereas strong agitation (120 rpm) of a conventional culture medium resulted in massive cell death, in the Bingham plastic fluid we obtained the same growth as the normal culture with optimal agitation (90 rpm). This indicates that Bingham plastic fluid protected cells from shear stress in suspension culture and could be used to enhance their robustness when developing a large‐scale.

中文翻译：

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Bingham 塑料液对人诱导多能干细胞悬浮培养中剪切应力的保护作用

悬浮培养是工业制备多能干细胞 (PSC)、再生治疗和药物筛选的重要方法。通常，悬浮培养需要搅拌以保持 PSC 聚集体悬浮并促进传质，但搅拌也会导致细胞损伤。在这项研究中，我们研究了使用添加了多糖基聚合物的 Bingham 塑料流体来保护 PSC 在悬浮培养中免受细胞损伤。流变分析表明，在添加聚合物 FP003 后，培养基获得了屈服应力并变成了 Bingham 塑性流体。生长/死亡分析表明，2 天的聚集体形成和 2 天的 Bingham 塑料培养基悬浮改善了细胞生长并防止了细胞死亡。在初始聚合步骤之后，虽然传统培养基的强烈搅拌（120 rpm）导致大量细胞死亡，但在 Bingham 塑料液中，我们在最佳搅拌（90 rpm）下获得了与正常培养相同的生长。这表明 Bingham 塑料流体在悬浮培养中保护细胞免受剪切应力，并且可用于在大规模开发时增强其稳健性。