Glycosylation of the reproductive tract of an adult female red‐necked ostrich (Struthio camelus camelus) carrying a fully formed calcified egg in her uterus when accidently killed by a blow to the head was examined using lectin histochemistry on samples from the infundibulum, magnum, uterus and vagina. Glycans in the luminal epithelium and underlying glands were described after staining with 23 lectins after neuraminidase pre‐treatment in some cases. Ciliated and non‐ciliated cells were evident at all levels in the luminal epithelium, the latter full of richly glycosylated secretory granules. The ciliated cells also showed glycosylation and, in the magnum, these cells often stained more intensely than the non‐ciliated cells. High mannose and complex N‐glycans, α1,6‐linked fucosyl and sialic acid residues were present throughout the tract and there was a complete absence of GalNAcα1,3(LFucα1,2)Galß1,3/4GlcNAcß1‐ and rare terminal GalNAcα1‐ residues. Fucose in α1,2‐linkage as H2 antigen and Le^y was also rare in the luminal epithelium and completely absent in glands. Terminal galactose was present in the luminal epithelium apart from in the infundibulum. Gland epithelium showed similar glycosylation to the luminal epithelium except in the magnum where there were significant differences and here the glands were packed full of large secretory granules, unlike the glands in the rest of the tract. Each section of the tract had its own specific pattern of glycosylation which could be related to the stage of egg formation.

中文翻译：

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鸵鸟（Struthio camelus camelus）生殖道糖基化的初步研究

成年雌性红颈鸵鸟（鸵鸟双峰驼）的生殖道糖基化）使用凝集素组织化学方法对漏斗，大瓶，子宫和阴道的样本进行检查，以检查发现被子宫头部意外撞击而在子宫中携带完全形成的钙化卵。在某些情况下，对神经氨酸酶预处理后用23种凝集素染色后，可以描述腔上皮和下腺中的聚糖。在管腔上皮细胞的各个水平上均可见纤毛和非纤毛细胞，后者充满了丰富的糖基化分泌颗粒。纤毛细胞也显示糖基化，在大瓶中，这些细胞的染色通常比非纤毛细胞更深。整个道中存在高甘露糖和复杂的N-聚糖，α1,6连接的岩藻糖基和唾液酸残基，并且完全不存在GalNAcα1,3（LFucα1,2）Galß1、3 / 4GlcNAcß1‐和罕见的末端GalNAcα1‐残基。^y在腔上皮中也很少见，在腺体中完全不存在。除漏斗外，末端半乳糖存在于腔上皮中。腺上皮显示出与管腔上皮相似的糖基化，除了在大瓶中存在显着差异外，此处腺体充满了大的分泌性颗粒，这与该导管其余部分的腺体不同。该道的每个部分都有其自己的特定糖基化模式，这可能与卵的形成阶段有关。