The goal was to investigate the relationship among mRNA expressions of anti‐Mullérian hormone (AMH), follicle‐stimulating hormone receptor (FSHR) and responses to superovulation (SO) in embryo donor dairy cows. Holstein cows (n = 19) were submitted to a standard SO protocol, with twice daily FSH treatments, and artificially inseminated. Prior to SO (Day 0), relative mRNA expressions of AMH and FSHR in blood were determined for all cows. Day 7 embryos were collected and were graded to determine superovulatory response for each donor. Results showed that relative mRNA expressions of AMH and FSHR were positively correlated (R² = 0.94). Relative mRNA expressions of both AMH and FSHR were positively correlated with total embryos (R² = 0.68 and 0.69, respectively), total transferable embryos (R² = 0.92 and 0.97, respectively) and total grade 1 embryos (R² = 0.54 and 0.59, respectively). Further, transcript abundances of AMH and FSHR positively associated with milk production of donor cows, and meanwhile, they were negatively associated with days in milk (DIM) at submission of cows to SO (p < .05) protocol. The relative mRNA expression of AMH was higher (p < .05) in donor cows <5 years of age. However, age of donor at superovulation did not influence mRNA expression of FSHR. Collectively, we infer that the mRNA expressions of AMH and FSHR prior to superovulation can predict donor cows’ positive response to superovulation.

中文翻译：

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抗穆勒氏激素和促卵泡激素受体的转录本丰富预测胚胎供体荷斯坦奶牛的超刺激反应

目的是研究胚胎供体奶牛中抗穆勒氏激素（AMH），卵泡刺激素受体（FSHR）的mRNA表达与超排卵反应（SO）之间的关系。将荷斯坦奶牛（n  = 19）接受标准SO方案，每天进行两次FSH处理，并人工授精。在SO（第0天）之前，确定所有奶牛血液中AMH和FSHR的相对mRNA表达。收集第7天的胚胎并分级以确定每个供体的超排卵反应。结果表明，AMH和FSHR的相对mRNA表达呈正相关（R ²  = 0.94）。AMH和FSHR的相对mRNA表达与胚胎总数正相关（R ² =分别为0.68和0.69），可移植胚胎总数（分别为R ²  = 0.92和0.97）和1级胚胎总数（ 分别为R ² = 0.54和0.59）。此外，AMH和FSHR的转录本丰度与供体母牛的产奶量呈正相关，与此同时，它们与母牛接受SO的牛奶天数（DIM）呈负相关（p  <.05）。 <5岁的供体母牛中，AMH的相对mRNA表达较高（p <.05）。然而，超排卵时供体的年龄并不影响FSHR的mRNA表达。总的来说，我们推测超排卵前AMH和FSHR的mRNA表达可以预测供体母牛对超排卵的阳性反应。