Tracing sources of carbon and hydrogen to stored lipids in the migratory moth, Mythimna unipuncta using stable isotopes (δ2H, δ13C),Physiological Entomology

当前位置： X-MOL 学术 › Physiol. Entomol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Tracing sources of carbon and hydrogen to stored lipids in the migratory moth, Mythimna unipuncta using stable isotopes (δ2H, δ13C)
Physiological Entomology ( IF 1.6 ) Pub Date : 2020-11-11 , DOI: 10.1111/phen.12343
Libesha Anparasan ₁ , Jeremy N. McNeil ₁ , Keith A. Hobson _{1,

2}

Affiliation

Stable isotope measurements of insect tissues can be used to determine origin and migratory patterns. The isotopic links between diet and stored lipids in laboratory reared true armyworm moths (Mythimna unipuncta, Haw.) were investigated using δ¹³C and δ²H measurements. Newly emerged moths were fed synthetic nectars, consisting of different carbohydrate and water sources that were isotopically distinct from those in the larval diet. After 4 days of feeding, insects were sacrificed, and fat‐body lipids were extracted for isotopic analysis. When held on a constant nectar source, adult diet contributed ∼87% of the C and 44% H of lipids. For H, 27% and ∼ 17% of lipid was derived from carbohydrates and water, respectively, with the remainder presumably from the larval stage. When the isotopic composition of the nectar source was switched there was rapid and exponential temporal change in lipid isotopic profiles. The relevance of our findings to tracing origins of stored lipids in migrant Lepidoptera is discussed.

中文翻译：

使用稳定的同位素（δ2H，δ13C）将碳和氢的来源追踪到迁移蛾（Mythimna unipuncta）中储存的脂质

昆虫组织的稳定同位素测量可用于确定起源和迁徙方式。在实验室饮食和存储脂质之间的同位素链接饲养真粘虫蛾（粘虫unipuncta，唧唧。），使用δ进行了调查¹³ C和δ ²H测量。新出现的飞蛾被喂食合成花蜜，这些花蜜由同位素和幼虫饮食不同的碳水化合物和水组成。喂食4天后，将昆虫处死，并提取脂肪脂质进行同位素分析。当使用恒定的花蜜来源时，成人饮食贡献了约87％的脂质C和44％的H脂质。对于H，脂质分别来自碳水化合物和水，分别占27％和约17％，其余部分大概来自幼体阶段。当切换花蜜来源的同位素组成时，脂质同位素分布迅速且呈指数性随时间变化。讨论了我们的发现与追踪鳞翅目迁徙鳞翅目中储存的脂质起源的相关性。

更新日期：2020-11-11

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11