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Rapid biosynthesis of fluorescent CdSe QDs in Bacillus licheniformis and correlative bacterial antibiotic change assess during the process
Luminescence ( IF 3.2 ) Pub Date : 2020-11-10 , DOI: 10.1002/bio.3980
Tian-Yang Lin 1, 2 , Zong-Juan Lian 1, 2 , Cai-Xia Yao 1, 2 , Qing-Qing Du 1, 2 , Sheng-Hua Liao 1, 2 , Sheng-Mei Wu 1, 2
Affiliation  

Cadmium selenide (CdSe) quantum dots (QDs) were biosynthesized rapidly in 18 h in Bacillus licheniformis ATCC 11946 (B. licheniformis); this process benefited from the cellular machinery of bacteria metal metabolism, in which inorganic Na2SeO3 and CdCl2 were chosen as raw materials to produce high quality CdSe QDs by a designed two‐step protocol. Research outcomes demonstrated that the purified CdSe QDs possessed maximum fluorescence intensities at weak alkalinity solutions and had good fluorescence stabilities at 4°C as well as at room temperature after standing for 1 week. Glutathione (GSH) concentration and superoxide dismutase (SOD) content, both of which were reported to be greatly related to biosynthetic activities in some bacterial matrices, were monitored during the biosynthetic process in B. licheniformis. Bacterial resistance research further showed that the change in rates in bacterial inhibition zone diameter to seven different antibiotics was less than 9% after B. licheniformis was used to manufacture CdSe QDs, showing a relative lower environmental risk in short‐term heavy metal exposure.

中文翻译:

地衣芽孢杆菌中荧光CdSe量子点的快速生物合成及过程中相关细菌抗生素变化的评估

地衣芽孢杆菌ATCC 11946(B. licheniformis)在18小时内快速生物合成了硒化镉(CdSe)量子点(QDs );这个过程得益于细菌金属代谢的细胞机制,其中无机Na 2 SeO 3和CdCl 2通过设计的两步协议,将其选择为生产高质量CdSe量子点的原料。研究结果表明,纯化的CdSe量子点在弱碱性溶液中具有最大的荧光强度,并且在放置1周后在4°C和室温下均具有良好的荧光稳定性。在地衣芽孢杆菌的生物合成过程中,监测了谷胱甘肽(GSH)浓度和超氧化物歧化酶(SOD)含量,两者均与某些细菌基质的生物合成活性密切相关。细菌耐药性研究进一步表明,地衣芽孢杆菌对七种不同抗生素的抑菌圈直径变化率小于9% 用于制造CdSe量子点,在短期重金属暴露中显示出相对较低的环境风险。
更新日期:2020-11-10
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