A selection method to develop an electrogenic consortium for improved power generation in microbial fuel cells (MFCs) is described. Raw domestic sewage was inoculated in a nutrient medium with Fe(III) hydroxide as the electron acceptor and subculturing was done. Subcultures were tested for Fe(III) reduction using ferrozine assay, microbial electron transfer activity using the Dye Reduction‐based Electron‐transfer Activity Monitoring (DREAM) assay and power generation in a MFC. Subcultures grown with Fe(III) demonstrated improvements in electron transfer activity and power generating ability, while cultures grown without Fe(III) did not exhibit these properties. The enriched Fe(III) subculture generated a maximum power density of 129.25 ± 7 mW/m², while the subculture grown without Fe(III) generated only 81.54 ± 8 mW/m². A high correlation was observed between DREAM assay and power generation (r = +0.963). Cyclic voltammetry corroborated the high electrochemical activity of the Fe‐enriched subculture. This method offers a simple and efficient way to enrich an electrogenic consortium for improved performance from routinely used inocula.

中文翻译：

---

增强放生性电联体以改善微生物燃料电池发电的选择策略

描述了开发用于改善微生物燃料电池（MFC）中的发电的电生财团的选择方法。将生活污水处理后，以氢氧化铁（III）作为电子受体，接种到营养培养基中，进行继代培养。使用亚铁分析测定亚培养液中的Fe（III）还原，使用基于染料还原的电子转移活性监测（DREAM）分析和MFC中的发电来测试微生物的电子转移活性。用Fe（III）生长的亚培养物表现出电子转移活性和发电能力的改善，而没有Fe（III）生长的培养物则没有这些特性。富集的Fe（III）亚培养产生的最大功率密度为129.25±7 mW / m ²，而没有Fe（III）的传代培养仅产生81.54±8 mW / m ²。在DREAM分析与发电之间观察到高度相关性（r = +0.963）。循环伏安法证实了富铁传代培养的高电化学活性。该方法提供了一种简单有效的方法来富集电生财团，以提高常规接种物中的性能。