An 8‐week growth trial was conducted to evaluate effects of dietary oil sources on growth, enzymes activity and genes expression levels related to lipid metabolism of hybrid grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatu) juveniles. Seven iso‐lipid (97 g/kg of dry matter) and iso‐protein (503.5 g/kg of dry matter) experimental diets were formulated containing 50 g/kg fish oil (FO; acting as controls) or various vegetable oils (VOs): corn oil (CO), sunflower oil (SO), tea oil (TO), olive oil (OO), rice oil (RO) and mixed oil (MO; comprising equal amounts of these oils). Each diet was fed to triplicate groups of 40 fish for per repetition (15.09 ± 0.01 g) for 56 days. The results show that (a) alternative dietary oils had no significant effects on final weight compared with control group (p > .05); (b) compared with FO group, VOs significantly changed the contents of serum lipoproteins, cholesterol, triglycerides and the activity of liver lipid‐metabolizing enzymes (p < .05); (c) CO group had the least effect on the serum lipoproteins, triglycerides and cholesterol of grouper compared with control; the activity of liver lipid‐metabolizing enzymes in RO and control group was the closest; (d) the mRNA levels of Δ6 Fatty acid desaturase (Δ6Fad), hormone‐sensitive lipase (HSL) and lipoprotein lipase (LPL) were not significantly effected by lipid sources, but CO, TO, OO and MO significantly down‐regulated the expression of fatty acid synthetase (FAS) mRNA level in liver, while RO opposite (p < .05); (e) vegetable oil significantly up‐regulated peroxisome proliferator‐activated receptor α (PPARα) and peroxisome proliferator‐activated receptor β (PPARβ) mRNA levels, while TO and RO down‐regulated peroxisome proliferator‐activated receptor γ (PPARγ) mRNA levels (p < .05); and 6) MO significantly increased the mRNA levels of heart‐type fatty acid‐binding protein (H‐FABP) and adipocyte‐type fatty acid‐binding protein (A‐FABP) (p < .05), while other VOs had no effect on them (p > .05). In conclusion, dietary substitution of FO by VO in diet affected lipid metabolism of grouper, which may be regulated by PPARs.

中文翻译：

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食用油对杂种石斑鱼脂代谢和相关基因表达的影响（♀Epinephelusfuscoguttatus×♂E。lanceolatu）

进行了为期8周的生长试验，以评估膳食油源对杂交石斑鱼（growth Epinephelus fuscoguttatus ×♂E . lanceolatu）脂质代谢相关的生长，酶活性和基因表达水平的影响。）少年。配制了7种异脂（97 g / kg干物质）和iso-蛋白质（503.5 g / kg干物质）实验日粮，其中含有50 g / kg鱼油（FO;作为对照）或各种植物油（VOs） ）：玉米油（CO），葵花子油（SO），茶油（TO），橄榄油（OO），米油（RO）和混合油（MO；包含等量的这些油）。将每种饮食喂入三组，每组40条鱼，每次重复（15.09±0.01 g），持续56天。结果表明：（a）替代饮食油与对照组相比对最终体重没有显着影响（p  > .05）；（b）与FO组相比，VOs显着改变了血清脂蛋白，胆固醇，甘油三酸酯和肝脂质代谢酶的活性（p <.05）; （c）与对照组相比，CO组对石斑鱼的血清脂蛋白，甘油三酸酯和胆固醇影响最小；RO组和对照组肝脂质代谢酶活性最接近。（d）脂质来源对Δ6脂肪酸去饱和酶（Δ6Fad），激素敏感性脂肪酶（HSL）和脂蛋白脂肪酶（LPL）的mRNA水平没有显着影响，但CO，TO，OO和MO显着下调了表达肝中脂肪酸合成酶（FAS）mRNA水平的变化，而RO则相反（p  <.05）；（e）植物油显着上调过氧化物酶体增殖物激活受体α（PPARα）和过氧化物酶体增殖物激活受体β（PPARβ）mRNA水平，而TO和RO下调过氧化物酶体增殖物激活受体γ（PPARγ）mRNA水平（p <.05）; 和6）MO显着增加了心脏型脂肪酸结合蛋白（H-FABP）和脂肪细胞型脂肪酸结合蛋白（A-FABP）的mRNA水平（p  <.05），而其他VOs没有影响在它们上（p  > .05）。总之，日粮中VO替代FO会影响石斑鱼的脂质代谢，这可能受PPAR调节。