Abstract Oligonucleotide aptamers are an important kind chemical antibody-like functional biomolecules. The aptamer-based mass spectrometric techniques can not only provide molecular mass and characteristic structures by mass spectrometry, but show high sensitivity and high affinity enrichment towards target molecules by aptamers. This review summarizes the advances in the aptamer- based mass spectrometric applications, and highlights the progress of aptamer-target interaction characterization, as well as the applications of aptamer as off-line or on-line affinity materials in mass spectrometric techniques. It suggests that the combination of various new mass spectrometric techniques, the construction or combination of varied pretreatment or on-line signal amplification approaches, especially the applications of aptameric functional nanomaterials, should be focused in the aspects of interaction characterization, selective extraction and highly sensitive detection of trace target molecules. The prospects of the development were also described.

中文翻译：

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寡核苷酸适体在质谱分析中的应用与展望

摘要 寡核苷酸适配体是一类重要的化学抗体样功能生物分子。基于适体的质谱技术不仅可以通过质谱提供分子质量和特征结构，而且可以通过适体对目标分子显示出高灵敏度和高亲和力。本综述总结了基于适配体的质谱应用的进展，并重点介绍了适配体-靶标相互作用表征的进展，以及适配体作为离线或在线亲和材料在质谱技术中的应用。它表明结合各种新的质谱技术，构建或组合各种预处理或在线信号放大方法，特别是适体功能纳米材料的应用，应重点关注痕量目标分子的相互作用表征、选择性提取和高灵敏度检测等方面。还介绍了发展前景。