Physicochemical, electrochemical and biological performance of 4 types of all‑carbon nanotube layers was studied. Higher oxidation state of carbon was responsible for micro-scaled uniformity of the layers and excellent electrical conductivity, while nitrogen containing functional groups yielded materials with anisotropy similar to natural tissues and reduced work function. All materials were cytocompatible with mammalian fibroblasts (viability >80%, cytotoxicity <3% at day 7) and human dermal fibroblast (viability of cells >70% at day 1), while reducing bacterial and cancer cells proliferation without adding any drug. After 8 h culture, a ~ 50% depletion in the number of Gram-positive bacteria was observed on materials with lower work function, while Gram-negative bacteria were more sensitive towards carbon coordination number and presence of nitrogen atoms (cell depletion of up to 48% on amidized carbon nanotubes). After 1-day culture, >80% reduction in the melanoma cells number, connected with enhanced production of reactive oxygen species (ROS) was observed. All‑carbon nanotube layers decreased bacteria and cancer cell functions without negatively influencing mammalian cells nor using drugs and we believe that this can be explained by various sensitivity of the tested cells towards exogenous ROS overproduction. As the concerns over implant-related infections as well as rates of antibiotic-resistant bacteria and chemotherapeutic-resistant cancer cells are growing, such materials should pave the way for a wide range of biomedical applications.

研究了4种全碳纳米管层的理化，电化学和生物性能。碳的较高氧化态负责各层的微观尺度均匀性和出色的导电性，而含氮官能团产生的材料具有与天然组织相似的各向异性，并降低了功函。所有材料均与哺乳动物成纤维细胞（在第​​7天的生存力> 80％，细胞毒性<3％）和人真皮成纤维细胞（在第​​1天的细胞生存力> 70％）具有细胞相容性，同时无需添加任何药物即可减少细菌和癌细胞的增殖。培养8小时后，在功函数较低的材料上观察到革兰氏阳性细菌数量减少了约50％，而革兰氏阴性细菌对碳配位数和氮原子的存在更为敏感（酰胺化碳纳米管的细胞耗竭高达48％）。培养1天后，观察到黑素瘤细胞数量减少> 80％，并与活性氧（ROS）产生增加有关。全碳纳米管层减少了细菌和癌细胞的功能，而不会对哺乳动物细胞产生负面影响，也没有使用药物，我们相信这可以通过被测细胞对外源性ROS过度产生的各种敏感性来解释。随着对植入物相关感染以及对抗生素耐药性细菌和对化疗耐药性癌细胞的比率的担忧日益增长，此类材料应为广泛的生物医学应用铺平道路。