Column selection often centers on the identification of a stationary phase that increases resolution for a certain class of compounds. While gains in resolution are most affected by selectivity of the stationary phase or modifications of the mobile phase, enhancements can still be made with an intentional selection of the packing material's microstructure. Unrestricted mass transfer into the particle's porous structure minimizes band broadening associated with hindered access to stationary phase. Increased efficiency, especially when operating above the optimal flow rates, can be gained if the pore size is significantly larger than the solvated analyte. Less studied are the effects of reduced access to pores due to physical hindrance and its impact on retention. This article explores the relationship between pore size and reversed phase retention, and specifically looks at a series of particle architectures with reversed phase and size exclusion modes to study retention associated with access to stationary phase surface area.

色谱柱选择通常集中在固定相的鉴定上，该固定相可提高某类化合物的分离度。虽然分辨率的提高受固定相选择性或流动相修改的影响最大，但仍然可以通过有意选择填充材料的微观结构来提高分辨率。不受限制地传质到颗粒的多孔结构中，最大限度地减少了与固定相进入受阻相关的谱带展宽。如果孔径明显大于溶剂化分析物，则可以提高效率，尤其是在高于最佳流速的情况下。较少研究是由于物理障碍而减少进入毛孔的影响及其对保留的影响。