N6-methyladeosine (m⁶A) plays an important role in virus infection and replication. Bombyx mori nuclear polyhedrosis is caused by Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Expression levels of m⁶A-modification-related genes after the infection of BmNPV were detected at first. Then, expression levels of BmNPV nucleocapsid protein gene VP39 and envelope fusion protein gene GP64 after knockdown of METTL3in vitro were quantified to identify the effect of m⁶A modification on BmNPV. BmNPV firstly infects the larval midgut in case of oral infection. Subsequently, to clarify the relationship between m⁶A modification and resistance of the silkworm to BmNPV, we detected the expression levels of m⁶A-modification-related genes invivo before and after infection of BmNPV. The results indicated that low METTL3 level hindered the proliferation of BmNPV to some extent, and silkworm strain with low METTL3 level showed stronger resistance against BmNPV. This study will accumulate new experimental data for elucidating the resistance mechanism of silkworm against BmNPV.

N6-甲基腺苷（m ⁶ A）在病毒感染和复制中起重要作用。家蚕核多角体病是由家蚕核多角体病毒（BmNPV）感染引起的。首先检测BmNPV感染后m ⁶ A-修饰相关基因的表达水平。然后，对体外敲除METTL 3后的BmNPV核衣壳蛋白基因VP 39和包膜融合蛋白基因GP 64的表达水平进行定量，以鉴定m ⁶的作用。对BmNPV的修改。口腔感染时，BmNPV首先感染幼虫中肠。随后，阐明米之间的关系⁶的修改和蚕的BmNPV的抗性，我们检测到m的表达水平⁶ A-修改相关基因INVIVO之前和多角体病毒的感染后。结果表明，低METTL3水平受阻的BmNPV的增殖到一定程度，并用低蚕应变METTL3水平显示出对多角体病毒的抗性强。这项研究将积累新的实验数据，以阐明家蚕抗BmNPV的机制。