Objective

To evaluate the effects of N,N'-dicyclohexylcarbodiimide (DCC), an ethanol-based dentin cross-linker, on the immediate and long-term microtensile bond strength (μTBS) and nanoleakage expression of a universal adhesive employed in self-etch mode (SE) or etch-and-rinse mode (ER). The effect of DCC on the dentinal MMP activity was also investigated by means of in-situ zymography.

Methods

Eighty freshly extracted human molars were sectioned to expose mid-coronal dentin surfaces. The teeth were assigned to one of the following groups, according to the dentin surface priming/adhesive approach: (G1): DCC pre-treatment and Scotchbond Universal (SBU) in ER mode; (G2): SBU in ER mode; (G3): DCC pretreatment and SBU in SE mode; (G4): SBU in SE mode. μTBS test was performed immediately (T₀) or after 1-year aging (T₁₂) in artificial saliva. Ten additional teeth per group were prepared for nanoleakage evaluation (N = 5) and for in-situ zymography (N = 5).

Results

Three-factor analysis of variance revealed significant difference for the variables DCC pretreatment, application mode and aging (p < 0.05) for both microtensile bond strength testing and in-situ zymography. Nanoleakage analysis revealed reduced marginal infiltration of DCC experimental groups both at T₀ and T₁₂.

Significance

The use of an ethanol-based primer containing DCC appears to be promising in preserving the stability of the adhesive interface of a universal adhesive, especially in the SE mode.

中文翻译：

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乙醇交联剂对通用胶粘剂的影响

目的

若要评估N，N'-二环己基碳二亚胺（DCC）（一种基于乙醇的牙本质交联剂）对以自蚀刻模式使用的通用粘合剂的近期和长期微拉伸粘合强度（μTBS）和纳米泄漏表达的影响（SE）或蚀刻漂洗模式（ER）。还通过原位酶谱法研究了DCC对牙本质MMP活性的影响。

方法

将80颗新鲜提取的人类臼齿切成段以暴露冠状中部的牙本质表面。根据牙本质表面打底/粘合方法，将牙齿分为以下一组：（G1）：ERC模式下的DCC预处理和Scotchbond Universal（SBU）；（G2）：处于ER模式的SBU；（G3）：在SE模式下进行DCC预处理和SBU；（G4）：SBU处于SE模式。立即在人工唾液中（T ₀）或老化1年后（T ₁₂）进行μTBS测试。每组准备十颗额外的牙齿用于纳漏评估（N = 5）和原位酶谱（N = 5）。

结果

三因素方差分析显示，DCC预处理变量，施加方式和时效（微拉伸键强度测试和原位酶谱分析）差异显着（p <0.05）。纳米渗漏分析显示DCC实验组在T ₀和T _12的边缘浸润减少。

意义

含有DCC的乙醇基底漆的使用似乎在保持通用粘合剂的粘合剂界面的稳定性方面很有希望，特别是在SE模式下。