Abstract Layered double hydroxide nanoparticles (LDH-NP) provide new therapeutic opportunities to improve drug distribution, stability, and effectivity. Nevertheless, these LDH-NP present a different behavior in biological medium due to their interaction with biomolecules (biological identity) to that of as-synthesized LDH-NP (synthetic identity). In this work, both identities were compared for LDH-NP functionalized with risedronate. First, physical interactions between as-synthesized LDH-NP and the cell membrane were studied using giant unilamellar vesicles (GUVs). Then, their biological identity was formed in bovine fetal serum and explored with a proteomic approach and the mapping of protein-protein interactions. Finally, the effect of functionalization and biological identity on drug delivery capacity was tested in methotrexate loaded LDH-NP. As a result of their opposite zeta potential, bare LDH-NP (ζ > +40 mV) increased the permeability of the negatively charged GUVs, while Ris functionalized LDH-NP (ζ

中文翻译：

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利塞膦酸盐功能化的层状双氢氧化物纳米载体的合成和生物学特性

摘要 层状双氢氧化物纳米粒子 (LDH-NP) 为改善药物分布、稳定性和有效性提供了新的治疗机会。然而，这些 LDH-NP 在生物介质中表现出不同的行为，因为它们与生物分子的相互作用（生物学特性）与合成的 LDH-NP（合成特性）的相互作用。在这项工作中，比较了用利塞膦酸盐功能化的 LDH-NP 的两种身份。首先，使用巨型单层囊泡 (GUVs) 研究合成的 LDH-NP 与细胞膜之间的物理相互作用。然后，在牛胎血清中形成了它们的生物学特性，并通过蛋白质组学方法和蛋白质-蛋白质相互作用图进行了探索。最后，在装载甲氨蝶呤的 LDH-NP 中测试了功能化和生物学特性对药物递送能力的影响。