Comparative profilings of cell growth and lipid production in the morphologically engineered strain (Δags1) and the wild type (WT) of Aspergillus oryzae BCC7051 were implemented. Using various nitrogen sources, a discrimination in cell morphology between the two strains was found, of which the Δags1 culture exhibited mycelial growth as small pellets in contrast to the WT. Of them, sodium nitrate and potassium nitrate were optimal for lipid production of the WT and Δags1 strains, respectively, which the highest lipid concentrations of 7.2 and 7.9 g L-1 were obtained in the respective cultures. The mathematical models of the growth kinetics and lipid phenotypes of both fungal strains were developed, enabling to distinguish three lipid-producing stages, including low lipid-producing, lipid accumulation, and lipid turnover stages. The model validation showed good performances in all nitrogen sources tested for the WT, but only NaNO3 and mixed yeast extract/NH4Cl were fitted well for the Δags1. The difference in the period of lipid-producing stages between the WT and Δags1 indicated the metabolic alterations of A. oryzae by the defect of a gene involved in the cell wall biosynthesis, which exhibited benefits for bioprocessing practices in addition to the high productivities of biomass and lipid. These findings would further permit the manipulation in the metabolic hub of the fungal production platform for other industrial purposes.

中文翻译：

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通过比较动力学模型探索米曲霉野生型和形态工程菌株产脂阶段的差异性状

对米曲霉 BCC7051 的形态工程菌株 (Δags1) 和野生型 (WT) 中的细胞生长和脂质生产进行了比较分析。使用各种氮源，发现两种菌株之间细胞形态的区别，其中 Δags1 培养物与 WT 相比，菌丝体生长为小颗粒。其中，硝酸钠和硝酸钾分别最适合 WT 和 Δags1 菌株的脂质生产，在各自的培养物中获得最高的脂质浓度为 7.2 和 7.9 g L-1。开发了两种真菌菌株的生长动力学和脂质表型的数学模型，能够区分三个脂质产生阶段，包括低脂质产生阶段、脂质积累阶段和脂质周转阶段。模型验证表明，在为 WT 测试的所有氮源中表现良好，但只有 NaNO3 和混合酵母提取物/NH4Cl 适合 Δags1。WT 和 Δags1 之间脂质产生阶段的差异表明米曲霉的代谢改变是由于参与细胞壁生物合成的基因缺陷导致的，除了生物质的高生产力外，这对生物加工实践也有好处和脂质。这些发现将进一步允许在真菌生产平台的代谢中心进行操作以用于其他工业目的。WT 和 Δags1 之间脂质产生阶段的差异表明米曲霉的代谢改变是由于参与细胞壁生物合成的基因缺陷导致的，除了生物质的高生产力外，这对生物加工实践也有好处和脂质。这些发现将进一步允许在真菌生产平台的代谢中心进行操作以用于其他工业目的。WT 和 Δags1 之间脂质产生阶段的差异表明米曲霉的代谢改变是由于参与细胞壁生物合成的基因缺陷导致的，除了生物质的高生产力外，这对生物加工实践也有好处和脂质。这些发现将进一步允许在真菌生产平台的代谢中心进行操作以用于其他工业目的。