Hybrid nucleic acid nanostructures partition architectural and functional roles between ribonucleic acid (RNA) joints and deoxyribonucleic acid (DNA) connectors. Nanoshapes self-assemble from nucleic acid modules through synergistic stabilization of marginally stable base pairing interactions within circularly closed polygons. Herein, we report the development of hybrid nanoshapes that include multiple different RNA modules such as internal loop and three-way junction (3WJ) motifs. An iterative mix-and-match screening approach was used to identify suitable DNA connectors that furnished stable nanoshapes for combinations of different RNA modules. The resulting complex multicomponent RNA-DNA hybrid nanoshapes were characterized by atomic force microscopy (AFM) imaging. Our research provides proof of concept for modular design, assembly and screening of RNA-DNA hybrid nanoshapes as building blocks for complex extended nucleic acid materials with features at the sub-10 nm scale.

杂种核酸纳米结构在核糖核酸（RNA）接头和脱氧核糖核酸（DNA）连接器之间分配了结构和功能。纳米形状通过在圆形封闭的多边形内边际稳定碱基配对相互作用的协同稳定作用，从核酸模块自组装。在本文中，我们报告了包括多个不同RNA模块（例如内部环和三向连接（3WJ）图案）的杂合纳米形状的发展。使用迭代混合匹配筛选方法来鉴定合适的DNA连接器，这些连接器可为不同RNA模块的组合提供稳定的纳米形状。通过原子力显微镜（AFM）成像对得到的复杂的多组分RNA-DNA杂合纳米形状进行了表征。我们的研究为模块化设计提供了概念验证，