Raspberry bushy dwarf virus (RBDV) is a long-known virus naturally infecting Rubus and grapevine. It is also one of the economically most important viruses of raspberries, but there are only a limited number of sequences covering a substantial part of the genome available in the databases. The aim of this study was: i) to study the geographic distribution of RBDV in Slovenia, and ii) to sequence RNA2 of several red raspberry and grapevine RBDV isolates and study their phylogeny and population structure. Geographic distribution studies were performed over a period of 13 years in three wine-growing regions of Slovenia (Primorska, Podravje and Posavje). The highest incidence of RBDV was found in Podravje (58.8%) and the lowest in Primorska (5.1%). Big differences were observed between Vipavska dolina (10.2%) and three other wine-growing districts of Primorska region (0.4–1.2%). Almost complete RNA2 sequences were obtained for four red raspberry isolates and seven grapevine isolates. Additionally, only coat protein sequences were obtained for three red raspberry isolates. Phylogenetic and population diversity analyses were performed on all available RBDV sequences. Phylogenetic analysis has shown clear differences in sequences from Rubus and grapevine that form two highly supported clades. In RNA2 analysis additional two sub-clades were found in grapevine clade. Two major subclades were identified also in the Rubus clade with further differentiation within these subclades. Purifying or stabilizing selection was found to be acting on both, CP and MP genes while few codons were found to be under positive selection.

覆盆子矮矮化病毒（RBDV）是一种自然感染Rubus的长期已知病毒和葡萄。它也是经济上最重要的覆盆子病毒之一，但数据库中覆盖基因组大部分的序列数量有限。这项研究的目的是：i）研究斯洛文尼亚RBDV的地理分布，以及ii）对几种红树莓和葡萄RBDV分离株的RNA2进行测序，并研究其系统发育和种群结构。在斯洛文尼亚的三个葡萄酒产区（Primorska，Podravje和Posavje）进行了13年的地理分布研究。RBDV发生率最高的是Podravje（58.8％），最低的是Primorska（5.1％）。在Vipavska dolina（10.2％）和滨海边疆区的其他三个葡萄酒产区（0.4–1.2％）之间观察到较大的差异。获得了四个红树莓分离物和七个葡萄树分离物的几乎完整的RNA2序列。另外，仅获得了三个红树莓分离物的外壳蛋白序列。在所有可用的RBDV序列上进行了系统发育和种群多样性分析。系统发育分析表明，与悬钩子和葡萄树形成两个高度支持的分支。在RNA2分析中，在葡萄进化枝中发现了另外两个亚进化枝。在Rubus进化枝中也鉴定出两个主要的进化枝，在这些进化枝中进一步分化。发现纯化或稳定选择对CP和MP基因都起作用，而发现很少的密码子处于正选择之下。