Methamphetamine (MA) and amphetamine (AM) are central nervous system doping agents, listed in the WADA Prohibited List, which in the past years showed the highest prevalence among all stimulants. Specifically, concerning these stimulants, it is essential to distinguish between d and l -isomers of AM and MA due to their distinction in illicit or legal nonprescription use. The main separation methods used for the urinary determination of MA and AM enantiomers are based on chromatographic procedures, such as gas chromatography after chiral derivatization or liquid chromatography (LC) applying chiral columns, followed by the mass spectrometer (MS) detection. The use of the optically pure reagent R -(-)-α-methoxy-α-(trifluoromethyl) phenylacetic acid chloride ( R -MTPA-Cl – Mosher’s Reagent) allows the isomeric separation by both chromatographic procedures while preserving the isomeric composition of the substances. Therefore, in the present work, an LC-MS 2 approach has been developed for AM and MA detection in urine after Mosher derivatization. The method was validated and can be used for the enantioselective confirmation of AM and MA for doping control purposes and forensic analysis in general, being useful for the distinction between the medicinal and illicit use of both stimulants.

中文翻译：

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使用 Mosher 衍生的液相色谱-串联质谱法对尿液中的苯丙胺和甲基苯丙胺进行手性分析

甲基苯丙胺 (MA) 和苯丙胺 (AM) 是中枢神经系统兴奋剂，被列入 WADA 禁用清单，在过去几年中显示出在所有兴奋剂中的流行率最高。具体来说，关于这些兴奋剂，必须区分 AM 和 MA 的 d 和 l 异构体，因为它们在非法或合法的非处方使用中有所不同。用于尿液测定 MA 和 AM 对映异构体的主要分离方法基于色谱程序，例如手性衍生化后的气相色谱或应用手性柱的液相色谱 (LC)，然后是质谱 (MS) 检测。使用光学纯试剂 R -(-)-α-甲氧基-α-(三氟甲基) 苯乙酸氯 (R -MTPA-Cl – Mosher 试剂) 允许通过两种色谱程序进行异构体分离，同时保留异构体的异构体组成物质。因此，在目前的工作中，开发了一种 LC-MS 2 方法，用于 Mosher 衍生化后尿液中的 AM 和 MA 检测。该方法经过验证，可用于 AM 和 MA 的对映选择性确认，用于兴奋剂控制目的和一般法医分析，可用于区分两种兴奋剂的药用和非法使用。