Liver dysfunction is a major health problem worldwide. Stem cells therapy has opened up new avenues for researches to treat liver diseases due to their multi lineage differentiation. As mesenchymal stem cells (MSCs) can be differentiated into hepatic lineages in the presence of different exogenous factors, the current study aimed to investigate the impact of carbon tetrachloride (CCl₄) induced liver injured mice serum on MSCs differentiation toward hepatocytes in vitro. Male Balb/c mice were treated for liver injury with CCl₄ as determined through biochemical tests spectrophotometrically and different growth factors (EGF, HGF) quantification through Sandwich ELISA in both normal and CCl₄-induced liver injured mice serum. Mice bone marrow derived-MSCs at second passage were treated with normal and CCl₄-induced liver injured mice serum. After 7 days, serum treated MSCs were investigated for hepatocytes like characteristics through RT-PCR. Serum biochemical tests (Bilirubin, ALT and ALP) and sandwich ELISA results of EGF and HGF showed marked increase in CCl₄ treated mice serum as compared to normal mice serum_. Periodic acid Schiff’s staining and urea assay kit confirmed high level of glycogen storage and urea production in cells treated with CCl₄-induced liver injured mice serum. RT-PCR results of CCl₄-induced liver injured mice serum treated cells also showed expression of hepatic markers (Albumin, Cyto-8, Cyto-18, and Cyto-19). This study confirmed that CCl₄-induced liver injured serum treatment can differentiate MSCs into hepatocyte-like cells in vitro.

肝功能障碍是世界范围内的主要健康问题。由于其多谱系分化，干细胞疗法为治疗肝脏疾病的研究开辟了新途径。由于间充质干细胞 (MSCs) 在不同外源性因素的存在下可以分化为肝谱系，目前的研究旨在研究四氯化碳 (CCl ₄ ) 诱导的肝损伤小鼠血清对体外 MSCs 向肝细胞分化的影响。用分光光度法生化测试确定的CCl ₄治疗雄性 Balb/c 小鼠的肝损伤，并通过夹心 ELISA 在正常和 CCl _{4 中}定量不同的生长因子（EGF、HGF）-诱导肝损伤小鼠血清。用正常和CCl ₄诱导的肝损伤小鼠血清处理第二代小鼠骨髓来源的MSC 。7天后，通过RT-PCR研究血清处理的MSC的肝细胞样特征。EGF 和 HGF 的血清生化测试（胆红素、ALT 和 ALP）和夹心 ELISA 结果显示，与正常小鼠血清相比，CCl ₄处理的小鼠血清显着增加_。高碘酸希夫氏染色和尿素测定试剂盒证实，在用 CCl ₄诱导的肝损伤小鼠血清处理的细胞中存在高水平的糖原储存和尿素产生。CCl _{4 的}RT-PCR结果诱导肝损伤小鼠血清处理的细胞也显示肝标志物（白蛋白、Cyto-8、Cyto-18 和 Cyto-19）的表达。本研究证实，CCl ₄诱导的肝损伤血清治疗可以在体外将MSCs分化为肝细胞样细胞。