Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against Gram-positive bacteria and fungus. It could effectively inhibit Staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of Staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer cell activities against several cancer cells via breaking the cell membrane of MGC803, HCT116 and C666-1 cancer cells.

抗菌肽的多种有益作用已引起越来越多的关注。在本研究中，从干酪乳杆菌HZ1的基因组中鉴定出一种新型AMP，其分子量为1875.5 Da 。该肽名为LHH1，由16个氨基酸残基组成，当溶于30 mM SDS中时，其α-螺旋含量为95.34％。LHH1对革兰氏阳性细菌和真菌表现出广泛的抗菌活性。它可以有效抑制金黄色葡萄球菌，最低抑菌浓度为3.5μM，并且溶血活性低。扫描电子显微镜，共聚焦激光扫描显微镜和流式细胞仪检测结果表明，LHH1通过破坏人肝癌细胞的细胞膜发挥其抗菌作用。金黄色葡萄球菌。同时，LHH1还通过破坏MGC803，HCT116和C666-1癌细胞的细胞膜表现出对几种癌细胞的抗癌细胞活性。