This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli.

本文研究了二硫氰基甲烷（DM）对大肠杆菌呼吸代谢中葡萄糖分解途径的抑制作用。。我们研究了DM对关键酶（ATPase和6磷酸葡萄糖脱氢酶G6PDH）的活性，关键产物（烟酰胺腺苷脱核苷酸氢磷酸NADPH）的水平以及己糖单磷酸途径中基因表达的影响。 （HMP）。结果表明，DM对被测菌株的最小抑菌浓度（MIC）和最小杀菌剂浓度（MBC）分别为5.86 mg / L和11.72 mg / L。与对照组中的细菌相比，在MIC中暴露于DM的细菌显示细菌ATPase和G6PDH活性，NADPH水平以及HMP途径中的基因表达增加，这可以解释为对DM引入的应激的行为反应。然而，大肠杆菌。