microRNAs (miRNA) families play a critical role in plant growth, development, and responses to abiotic stress. In this study, we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress. The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated. N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes, including cyclin A3 and cyclin L, which were strictly required for cell cycle progression. In addition, U. prolifera with highly expression of Up-miR-843 showed improved biomass, and photosynthesis compared with that under normal growth conditions. Thus, the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes, which further regulated the growth of U. prolifera.

microRNA（miRNA）家族在植物生长，发育以及对非生物胁迫的反应中起关键作用。在这项研究中，我们表征了Up-miR-843及其靶基因在Ulva增殖中对氮退化和热胁迫的反应。数据表明可以成功验证Up-miR-843的184个靶基因。氮缺乏而不是热应激刺激诱导了Up-miR-843丰度的增加，同时显示了靶基因的反向表达，包括细胞周期蛋白的严格表达，包括细胞周期蛋白A3和细胞周期蛋白L。此外，U。prolifera与正常生长条件下相比，具有高表达Up-miR-843的蛋白具有改善的生物量和光合作用。因此，N缺乏和热响应性miRNA可能是介导这些靶基因表达的可能成员，这进一步调节了U. prolifera的生长。