The aim of this work was to study the cytotoxicity of Ti–6Al–4V–5Cu (TC4–Cu) alloy for dental applications and evaluate the cell viability of different fabricated TC4–Cu alloys in contact with MC3T3-E1 cells in vitro. Ti–6Al–4V (TC4) alloy was used as a negative control to evaluate the cytotoxicity level of TC4–Cu alloy so as to provide basic support for the dental clinical application. Control group TC4 and experimental group TC4–Cu with different fabrications were incubated in the cell culture medium. The absorbance value of mouse osteoblast MC3T3-E1 cells was detected by CCK-8 assay after 1, 3 and 7 days, respectively. The relative proliferation rate of cells was calculated, and then the toxicity level was valued for each group. Cell morphology on the surface was also studied by observing the cytoskeleton through F-actin filament staining. The experimental results showed that the absorbance values for the experimental and the negative control groups were not same at different time points. Compared with different fabricated TC4–Cu alloys, the annealing-TC4–Cu alloy showed much better biocompatibility. The mouse osteoblast MC3T3-E1 cells cultured with annealing-TC4–Cu, rolling-TC4–Cu and solution + aging-TC4–Cu have no toxic effects, and these alloys could promote the proliferation of mouse osteoblasts.

这项工作的目的是研究用于牙科应用的Ti-6Al-4V-5Cu（TC4-Cu）合金的细胞毒性，并评估与体外MC3T3-E1细胞接触的不同TC4-Cu合金的细胞生存能力。Ti-6Al-4V（TC4）合金用作阴性对照，以评估TC4-Cu合金的细胞毒性水平，从而为牙科临床应用提供基本支持。在细胞培养基中孵育具有不同结构的对照组TC4和实验组TC4–Cu。1、3、7天后分别通过CCK-8法检测小鼠成骨细胞MC3T3-E1的吸光度值。计算细胞的相对增殖率，然后评估每组的毒性水平。还通过F-肌动蛋白丝染色观察细胞骨架来研究表面的细胞形态。实验结果表明，实验组和阴性对照组在不同时间点的吸光度值不相同。与不同的TC4–Cu合金相比，TC4–Cu退火合金具有更好的生物相容性。用退火TC4–Cu，滚动TC4–Cu和溶液+老化TC4–Cu培养的小鼠成骨细胞MC3T3-E1细胞没有毒性作用，这些合金可以促进小鼠成骨细胞的增殖。