Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Determination of the binding site size of hexaammineruthenium(III) inside monolayers of DNA on gold
Analyst ( IF 3.6 ) Pub Date : 2020-10-28 , DOI: 10.1039/d0an01685c Alireza Abi 1, 2, 3, 4, 5 , Afsaneh Safavi 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2020-10-28 , DOI: 10.1039/d0an01685c Alireza Abi 1, 2, 3, 4, 5 , Afsaneh Safavi 1, 2, 3, 4, 5
Affiliation
|
Hexaammineruthenium(III), RuHex3+, is a DNA-binding metal complex that is widely used as a redox marker for the indirect determination of DNA coverage at the electrode surface. The conversion of electrochemically quantifiable surface excess of RuHex3+ into DNA surface coverage requires the knowledge of the binding site size of RuHex3+ (s). Traditionally, s on surface-immobilized DNA has been assumed to be equivalent to that on solution-phase DNA, which was experimentally determined in previous studies. Nevertheless, the different local microenvironments existing inside DNA monolayers in comparison to that in bulk solutions cast doubt on the validity of this assumption. In this report, we used electrochemical techniques to investigate s on surface-immobilized DNA. The values of s inside the DNA monolayers were found to be significantly smaller than that reported on solution-phase DNA. Besides, s was found to depend on the DNA packing density and became larger by increasing the DNA surface coverage or hybridizing the surface-tethered DNAs with complementary strands. Our data indicate that the RuHex3+ method, in which an s value of 3 nucleotides is used for the conversion of RuHex3+ to DNA surface coverage, does not always give reliable results.
中文翻译：
黄金上DNA单层内六氨合钌（III）结合位点大小的测定
六氨合钌（III），RuHex 3+，是一种结合DNA的金属络合物，被广泛用作间接确定电极表面DNA覆盖度的氧化还原标记。RuHex 3+的电化学可量化的表面过量转化为DNA表面覆盖率，需要了解RuHex 3+（s）的结合位点大小。传统上，s表面固定DNA上的DNA假定与溶液相DNA上的相同，这是先前研究中通过实验确定的。然而，与本体溶液相比，DNA单分子层内部存在的不同局部微环境使这一假设的有效性受到怀疑。在这份报告中，我们采用电化学技术研究小号上表面固定DNA。发现DNA单层内部的s值明显小于溶液相DNA报道的s值。此外，发现s取决于DNA堆积密度，并通过增加DNA表面覆盖率或将表面束缚的DNA与互补链杂交而变大。我们的数据表明RuHex3+方法（其中3个核苷酸的s值用于RuHex 3+到DNA表面覆盖的转化）并不总是能提供可靠的结果。
更新日期：2020-11-09
中文翻译：
黄金上DNA单层内六氨合钌（III）结合位点大小的测定
六氨合钌（III），RuHex 3+，是一种结合DNA的金属络合物，被广泛用作间接确定电极表面DNA覆盖度的氧化还原标记。RuHex 3+的电化学可量化的表面过量转化为DNA表面覆盖率，需要了解RuHex 3+（s）的结合位点大小。传统上，s表面固定DNA上的DNA假定与溶液相DNA上的相同，这是先前研究中通过实验确定的。然而，与本体溶液相比，DNA单分子层内部存在的不同局部微环境使这一假设的有效性受到怀疑。在这份报告中，我们采用电化学技术研究小号上表面固定DNA。发现DNA单层内部的s值明显小于溶液相DNA报道的s值。此外，发现s取决于DNA堆积密度，并通过增加DNA表面覆盖率或将表面束缚的DNA与互补链杂交而变大。我们的数据表明RuHex3+方法（其中3个核苷酸的s值用于RuHex 3+到DNA表面覆盖的转化）并不总是能提供可靠的结果。
京公网安备 11010802027423号