Porous materials containing cells—prepared via cell seeding on scaffolds or gelation of cell-containing solutions—have been widely studied to investigate tissue regeneration and three-dimensional cultures. However, these methods cannot introduce cells into porous materials that have low water absorption or scaffolds that require cytotoxic solvents or processes for their production. In this study, first, three different impregnation treatments conditions (vacuum, pressure, and vacuum pressure impregnation: VPI) were applied to cell suspensions to evaluate the effect of each treatment on cells. Following all three treatments, fibroblasts adhered to the cell culture dish and proliferated in the same manner as untreated cells, which confirmed that the three impregnation treatments did not affect cell function. Second, cells were introduced into a poly-l-lactic acid (PLA) scaffold, which has low water absorption, using the same impregnation treatments. The PLA scaffolds subjected to the three impregnation treatments that exhibited a significantly greater amount of DNA than those subjected to immersion treatments and showed increasing amounts of DNA in the order vacuum treatment > VPI treatment > pressure treatment. Furthermore, the amount of DNA in the vacuum-treated and VPI-treated PLA scaffolds increased on the first, third, and fifth days of culture, and it was confirmed that the cells introduced into the PLA scaffolds proliferated. These results suggest that vacuum and VPI treatments may be useful methods for introducing cells into porous materials.

中文翻译：

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使用浸渍技术将细胞引入多孔聚-l-乳酸支架

含有细胞的多孔材料——通过在支架上接种细胞或含细胞溶液的凝胶化制备——已被广泛研究以研究组织再生和三维培养。然而，这些方法不能将细胞引入吸水率低的多孔材料或需要细胞毒性溶剂或生产工艺的支架。在本研究中，首先，将三种不同的浸渍处理条件（真空、压力和真空压力浸渍：VPI）应用于细胞悬液，以评估每种处理对细胞的影响。在所有三种处理之后，成纤维细胞粘附到细胞培养皿上并以与未处理细胞相同的方式增殖，这证实了三种浸渍处理不影响细胞功能。其次，将细胞引入聚l -乳酸 (PLA) 支架，吸水率低，使用相同的浸渍处理。经过三种浸渍处理的 PLA 支架显示出比经过浸渍处理的那些显着更多的 DNA 量，并显示出 DNA 量增加的顺序真空处理 > VPI 处理 > 压力处理。此外，真空处理和 VPI 处理的 PLA 支架中的 DNA 量在培养的第一天、第三天和第五天增加，并证实导入 PLA 支架的细胞增殖。这些结果表明真空和 VPI 处理可能是将细胞引入多孔材料的有用方法。